Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 535926
Title Cloning and Functional Analysis of three Cold Regulated CBF Genes in the Overwintering Crucifer Boechera stricta
Author(s) Heo, Jaeyun; Tienderen, Peter van; Schranz, M.E.
Source International Journal of Agriculture & Biology 20 (2018)3. - ISSN 1560-8530 - p. 594 - 600.
DOI https://doi.org/10.17957/IJAB/15.0528
Department(s) Biosystematics
EPS
Publication type Refereed Article in a scientific journal
Publication year 2018
Abstract In this research, we isolated three CBF (C-repeat-Binding Factors) genes from two genotypes of Boechera stricta with contrasting freezing tolerance and characterized their structure and expression patterns in response to cold treatment. An amino acid sequence comparison revealed that the CBF genes in B. stricta showed high conservation in the AP2 domain and PKKP/RAGR motif like other cold adaptable Brassicaceae. The pairwise sequence alignment of the CBF genes isolated from two genotypes of B. stricta showed non-synonymous mutations in CBF 2 and 3. Gene expression analysis demonstrated that
CBF genes in B. stricta have expression patterns similar to CBFs in A. thaliana in response to cold treatment, while differential expression at the molecular level in CBF and COR genes was presented between two genotypes of B. stricta. Our
results suggest that signal transduction of three CBF genes can be one of the central pathways in the development of freezing tolerance in B. stricta.
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