Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 536167
Title Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8
Author(s) Jiang, Rui; Li, Jingcai; Tian, Zhendong; Du, Juan; Armstrong, Miles; Baker, Katie; Tze-Yin Lim, Joanne; Vossen, Jack H.; He, Huan; Portal, Leticia; Zhou, Jun; Bonierbale, Merideth; Hein, Ingo; Lindqvist-Kreuze, Hannele; Xie, Conghua
Source Journal of Experimental Botany 69 (2018)7. - ISSN 0022-0957 - p. 1545 - 1555.
DOI https://doi.org/10.1093/jxb/ery021
Department(s) WUR PB Resistance in Solanaceae
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) dRenSeq - field resistance - late blight - map-based cloning - potato - R gene
Abstract Following the often short-lived protection that major nucleotide binding, leucine-rich-repeat (NB-LRR) resistance genes offer against the potato pathogen Phytophthora infestans, field resistance was thought to provide a more durable alternative to prevent late blight disease. We previously identified the QTL dPI09c on potato chromosome 9 as a more durable field resistance source against late blight. Here, the resistance QTL was fine-mapped to a 186 kb region. The interval corresponds to a larger, 389 kb, genomic region in the potato reference genome of Solanum tuberosum Group Phureja doubled monoploid clone DM1-3 (DM) and from which functional NB-LRRs R8, R9a, Rpi-moc1, and Rpi-vnt1 have arisen independently in wild species. dRenSeq analysis of parental clones alongside resistant and susceptible bulks of the segregating population B3C1HP showed full sequence representation of R8. This was independently validated using long-range PCR and screening of a bespoke bacterial artificial chromosome library. The latter enabled a comparative analysis of the sequence variation in this locus in diverse Solanaceae. We reveal for the first time that broad spectrum and durable field resistance against P. infestans is conferred by the NB-LRR gene R8, which is thought to provide narrow spectrum race-specific resistance.
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