Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 536348
Title Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation
Author(s) Vreman, Sandra; Auray, Gael; Savelkoul, Huub F.J.; Rebel, Annemarie; Summerfield, Artur; Stockhofe-Zurwieden, Norbert
Source Developmental and Comparative Immunology 84 (2018). - ISSN 0145-305X - p. 361 - 370.
Department(s) CVI Infection Biology
Cell Biology and Immunology
LR - Animal Behaviour & Welfare
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Dendritic cell - Innate immunity - Neonate - Porcine - Toll like receptor ligand
Abstract The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14−CD4+CD172a+CADM1-) and conventional DCs (cDCs), being further divided into a cDC1 (CD14−CD4−CD172alowCADM1+) and a cDC2 (CD14−CD4−CD172a+CADM1+) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.
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