Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 536386
Title Converting Escherichia coli into an archaebacterium with a hybrid heterochiral membrane
Author(s) Caforio, Antonella; Siliakus, Melvin F.; Exterkate, Marten; Jain, Samta; Jumde, Varsha R.; Andringa, Ruben L.H.; Kengen, Servé W.M.; Minnaard, Adriaan J.; Driessen, Arnold J.M.; Oost, John van der
Source Proceedings of the National Academy of Sciences of the United States of America 115 (2018)14. - ISSN 0027-8424 - p. 3704 - 3709.
DOI https://doi.org/10.1073/pnas.1721604115
Department(s) Microbiological Laboratory
VLAG
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Archaea - Bacteria - Ether lipids - Hybrid membranes - Lipid biosynthesis
Abstract One of the main differences between bacteria and archaea concerns their membrane composition. Whereas bacterial membranes are made up of glycerol-3-phosphate ester lipids, archaeal membranes are composed of glycerol-1-phosphate ether lipids. Here, we report the construction of a stable hybrid heterochiral membrane through lipid engineering of the bacterium Escherichia coli. By boosting isoprenoid biosynthesis and heterologous expression of archaeal ether lipid biosynthesis genes, we obtained a viable E. coli strain of which the membranes contain archaeal lipids with the expected stereochemistry. It has been found that the archaeal lipid biosynthesis enzymes are relatively promiscuous with respect to their glycerol phosphate backbone and that E. coli has the unexpected potential to generate glycerol-1-phosphate. The unprecedented level of 20–30% archaeal lipids in a bacterial cell has allowed for analyzing the effect on the mixed-membrane cell’s phenotype. Interestingly, growth rates are unchanged, whereas the robustness of cells with a hybrid heterochiral membrane appeared slightly increased. The implications of these findings for evolutionary scenarios are discussed.
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