Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 536528
Title Chemical characterization and wound healing property of a β-D-glucan from edible mushroom Piptoporus betulinus
Author(s) Jesus, Liana Inara de; Smiderle, Fhernanda R.; Ruthes, Andrea C.; Vilaplana, Francisco; Lin, Fernando Tonholi Dal'; Maria-Ferreira, Daniele; Werner, Maria Fernanda; Griensven, Leo J.L.D. Van; Iacomini, Marcello
Source International Journal of Biological Macromolecules 117 (2018). - ISSN 0141-8130 - p. 1361 - 1366.
DOI https://doi.org/10.1016/j.ijbiomac.2017.12.107
Department(s) PRI BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Random coil - Wound healing - β-D-glucan
Abstract A water-soluble β-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 × 105 Da with a random coil conformation for molecular weights below 1 × 106 Da. Typical signals of β-(1 → 3)-linkages were observed in NMR spectrum (δ 102.7/4.76; 102.8/4.74; 102.9/4.52; and δ 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at δ 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a β-D-glucan with a main chain (1 → 3)-linked, substituted at O-6 by single-units of glucose. The β-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 μg mL-1 and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.
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