Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 536732
Title Survival of Ralstonia solanacearum and Ralstonia pseudosolanacearum in drain water
Author(s) Stevens, L.H.; Zouwen, P.S. van der; Tongeren, C.A.M. van; Kastelein, P.; Wolf, J.M. van der
Source EPPO Bulletin 48 (2018)1. - ISSN 0250-8052 - p. 97 - 104.
Department(s) PPO/PRI Biointeractions and Plant Health
Publication type Refereed Article in a scientific journal
Publication year 2018
Abstract The survival in drain water of two strains of Ralstonia solanacearum and three strains of Ralstonia pseudosolanacearum, including two strains able to cause wilt in roses, was determined. Water draining from drip-irrigated rock wool mats on which roses were grown was supplemented with the pathogen and survival was monitored at 4, 12, 20 and 28°C for up to 112 days. All strains were able to survive for at least 112 days in drain water at 12 and 20°C, but at 4°C maximum survival was 56 days. At 28°C, the survival period was strain dependent, but was at least 56 days. Populations declined gradually in non-sterile drain water to a low level (maximum 100 cfu mL−1 after 112 days). In sterile drain water (autoclaved prior to addition of populations), no or only a limited decline in populations was found at 112 days, dependent on strain and temperature. Drain water that tested negative for Ralstonia in the dilution plating assay was tested for the presence of cells in a viable but non-culturable state (VBNCs). Tomato plants were inoculated, but no symptoms developed, and plants sampled 22 days post-inoculation were negative in a plating assay. Therefore, no indications were found that VBNCs were present.
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