Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 537548
Title Fractionation of proteins and carbohydrates from crude microalgae extracts using an ionic liquid based-aqueous two phase system
Author(s) Suarez Garcia, Edgar; Suarez Ruiz, Catalina A.; Tilaye, Tewodros; Eppink, Michel H.M.; Wijffels, Rene H.; Berg, Corjan van den
Source Separation and Purification Technology 204 (2018). - ISSN 1383-5866 - p. 56 - 65.
DOI https://doi.org/10.1016/j.seppur.2018.04.043
Department(s) Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Crude protein - Ionic liquid - Partitioning - Phase diagram - Tie lines
Abstract

Mild, simple and efficient recovery methods are required to obtain high-value microalgae proteins. As a promising extraction method, an Aqueous two phase system (ATPS) was used to partition proteins from crude microalgae extracts obtained from two green microalgae of industrial interest: Neochloris oleoabundans and Tetraselmis suecica. Furthermore, the Non-Random Two Liquids model (NRTL) was applied to describe both the phase diagram and the partition coefficient of total protein. It was observed that total protein preferentially concentrates in the top phase. Additionally, no significant effect on partition or extraction efficiency was noted at different tie lines. Experimental data indicate that proteins and sugars are selectively fractionated in top and bottom phases respectively. The model provided a good representation of the experimental data for the liquid-liquid equilibrium. Moreover, the model also led to a good representation of the partitioning data for two reference proteins, Rubisco and Bovine Serum Albumin (BSA), as well as for total protein from crude microalgae extracts.

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