Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 537962
Title Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform
Author(s) Kinahan, David J.; Julius, Lourdes A.N.; Schoen, Cor; Dreo, Tanja; Ducree, Jens
Source In: 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. - Institute of Electrical and Electronics Engineers Inc. - ISBN 9781538647820 - p. 1134 - 1137.
Event 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, 2018-01-21/2018-01-25
DOI https://doi.org/10.1109/MEMSYS.2018.8346761
Department(s) PPO/PRI Biointeractions and Plant Health
Publication type Contribution in proceedings
Publication year 2018
Abstract This work presents a rotational-pulse actuated micro-fluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated 'Lab-on-a-Disc' (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using 'shake mode' agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.
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