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Record number 538039
Title High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei
Author(s) Girault, G.; Wattiau, P.; Saqib, M.; Martin, B.; Vorimore, F.; Singha, H.; Engelsma, M.; Roest, H.J.; Spicic, S.; Grunow, R.; Vicari, N.; Keersmaecker, S.C.J. De; Roosens, N.H.C.; Fabbi, M.; Tripathi, B.N.; Zientara, S.; Madani, N.; Laroucau, K.
Source Infection, genetics and evolution 63 (2018). - ISSN 1567-1348 - p. 1 - 4.
Department(s) CVI Diagnostics and Crisis
CVI Bacteriology and Epidemiology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Burkholderia mallei - Genotyping - HRM

Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.

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