Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 538039
Title High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei
Author(s) Girault, G.; Wattiau, P.; Saqib, M.; Martin, B.; Vorimore, F.; Singha, H.; Engelsma, M.; Roest, H.J.; Spicic, S.; Grunow, R.; Vicari, N.; Keersmaecker, S.C.J. De; Roosens, N.H.C.; Fabbi, M.; Tripathi, B.N.; Zientara, S.; Madani, N.; Laroucau, K.
Source Infection, genetics and evolution 63 (2018). - ISSN 1567-1348 - p. 1 - 4.
DOI https://doi.org/10.1016/j.meegid.2018.05.004
Department(s) CVI Diagnostics and Crisis
CVI Bacteriology and Epidemiology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Burkholderia mallei - Genotyping - HRM
Abstract

Burkholderia (B.) mallei is the causative agent of glanders. A previous work conducted on single-nucleotide polymorphisms (SNP) extracted from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically informative SNPs within the genome of B. mallei that subtype the species into 3 lineages and 12 branches/sub-branches/groups. The present results demonstrate that SNP-based genotyping represent an interesting approach for the molecular epidemiology analysis of B. mallei.

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