Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 538194
Title Host- and stage-dependent secretome of the arbuscular mycorrhizal fungus Rhizophagus irregularis
Author(s) Zeng, Tian; Holmer, Rens; Hontelez, Jan; Lintel-Hekkert, Bas te; Marufu, Lucky; Zeeuw, Thijs de; Wu, Fangyuan; Schijlen, Elio; Bisseling, Ton; Limpens, Erik
Source The Plant Journal 94 (2018)3. - ISSN 0960-7412 - p. 411 - 425.
DOI https://doi.org/10.1111/tpj.13908
Department(s) Laboratory of Molecular Biology
Bioinformatics
PRI BIOS Applied Bioinformatics
Publication type Refereed Article in a scientific journal
Publication year 2018
Abstract Arbuscular mycorrhizal fungi form the most wide‐spread endosymbiosis with plants. There is very little host‐specificity in this interaction, however host preferences as well as varying symbiotic efficiencies have been observed. We hypothesize that secreted proteins (SPs) may act as fungal effectors to control symbiotic efficiency in a host‐dependent manner. Therefore, we studied whether AM fungi adjust their secretome in a host‐ and stage‐dependent manner to contribute to their extremely wide host‐range. We investigated the expression of SP‐encoding genes of Rhizophagus irregularis in three evolutionary distantly‐related plant species, Medicago truncatula, Nicotiana benthamiana and Allium schoenoprasum. In addition we used laser microdissection in combination with RNAseq to study SP expression at different stages of the interaction in Medicago. Our data indicate that most expressed SPs show roughly equal expression levels in the interaction with all three host plants. In addition, a subset shows significant differential expression depending on the host plant. Furthermore, SP expression is controlled locally in the hyphal network in response to host dependent cues. Overall, this study presents a comprehensive analysis of the R. irregularis secretome, which now offers a solid basis to direct functional studies on the role of fungal SPs in AM symbiosis.
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