Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 538205
Title Bidirectional backcrosses between wild and cultivated lettuce identify loci involved in nonhost resistance to downy mildew
Author(s) Giesbers, Anne K.J.; Boer, Erik Den; Braspenning, David N.J.; Bouten, Thijs P.H.; Specken, Johan W.; Kaauwen, Martijn P.W. van; Visser, Richard G.F.; Niks, Rients E.; Jeuken, Marieke J.W.
Source Theoretical and Applied Genetics 131 (2018)8. - ISSN 0040-5752 - p. 1761 - 1776.
DOI https://doi.org/10.1007/s00122-018-3112-8
Department(s) Laboratory of Plant Breeding
Team Internationale Productie & Gewasinnovatie
WUR PB Non host en Insectenresistentie
PE&RC
EPS
PBR Non host en Insectenresistentie
Publication type Refereed Article in a scientific journal
Publication year 2018
Abstract

Key message: The nonhost resistance of wild lettuce to lettuce downy mildew seems explained by four components of a putative set of epistatic genes. Abstract: The commonplace observation that plants are immune to most potential pathogens is known as nonhost resistance (NHR). The genetic basis of NHR is poorly understood. Inheritance studies of NHR require crosses of nonhost species with a host, but these crosses are usually unsuccessful. The plant-pathosystem of lettuce and downy mildew, Bremia lactucae, provides a rare opportunity to study the inheritance of NHR, because the nonhost wild lettuce species Lactuca saligna is sufficiently cross-compatible with the cultivated host Lactuca sativa. Our previous studies on NHR in one L. saligna accession led to the hypothesis that multi-locus epistatic interactions might explain NHR. Here, we studied NHR at the species level in nine accessions. Besides the commonly used approach of studying a target trait from a wild donor species in a cultivar genetic background, we also explored the opposite, complementary approach of cultivar introgression in a wild species background. This bidirectional approach encompassed (1) nonhost into host introgression: identification of L. saligna derived chromosome regions that were overrepresented in highly resistant BC1 plants (F1 × L. sativa), (2) host into nonhost introgression: identification of L. sativa derived chromosome regions that were overrepresented in BC1 inbred lines (F1 × L. saligna) with relatively high infection levels. We demonstrated that NHR is based on resistance factors from L. saligna and the genetic dose for NHR differs between accessions. NHR seemed explained by combinations of epistatic genes on three or four chromosome segments, of which one chromosome segment was validated by the host into nonhost approach.

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