Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 538380
Title Evaluation of ELISA and haemagglutination inhibition as screening tests in serosurveillance for H5/H7 avian influenza in commercial chicken flocks
Author(s) Arnold, M.E.; Slomka, M.J.; Breed, A.C.; Hjulsager, C.K.; Pritz-Verschuren, S.; Venema-Kemper, S.; Bouwstra, R.J.; Trebbien, R.; Zohari, S.; Ceeraz, V.; Larsen, L.E.; Manvell, R.J.; Koch, G.; Brown, I.H.
Source Epidemiology and Infection 146 (2018)3. - ISSN 0950-2688 - p. 306 - 313.
DOI https://doi.org/10.1017/S0950268817002898
Department(s) CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) AIV (avian influenza virus) - Bayesian - ELISA (enzyme-linked immunosorbent assay) - H5/H7 AIV surveillance - HI (haemagglutination inhibition)
Abstract

Avian influenza virus (AIV) subtypes H5 and H7 can infect poultry causing low pathogenicity (LP) AI, but these LPAIVs may mutate to highly pathogenic AIV in chickens or turkeys causing high mortality, hence H5/H7 subtypes demand statutory intervention. Serological surveillance in the European Union provides evidence of H5/H7 AIV exposure in apparently healthy poultry. To identify the most sensitive screening method as the first step in an algorithm to provide evidence of H5/H7 AIV infection, the standard approach of H5/H7 antibody testing by haemagglutination inhibition (HI) was compared with an ELISA, which detects antibodies to all subtypes. Sera (n = 1055) from 74 commercial chicken flocks were tested by both methods. A Bayesian approach served to estimate diagnostic test sensitivities and specificities, without assuming any ‘gold standard’. Sensitivity and specificity of the ELISA was 97% and 99.8%, and for H5/H7 HI 43% and 99.8%, respectively, although H5/H7 HI sensitivity varied considerably between infected flocks. ELISA therefore provides superior sensitivity for the screening of chicken flocks as part of an algorithm, which subsequently utilises H5/H7 HI to identify infection by these two subtypes. With the calculated sensitivity and specificity, testing nine sera per flock is sufficient to detect a flock seroprevalence of 30% with 95% probability.

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