Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 538501
Title Integrating quantitative morphological and qualitative molecular methods to analyse soil nematode community responses to plant range expansion
Author(s) Geisen, Stefan; Snoek, L.B.; Hooven, Freddy C. ten; Duyts, Henk; Kostenko, Olga; Bloem, Janneke; Martens, Henk; Quist, Casper W.; Helder, Johannes A.; Putten, Wim H. van der
Source Methods in Ecology and Evolution 9 (2018)6. - ISSN 2041-210X - p. 1366 - 1378.
DOI https://doi.org/10.1111/2041-210X.12999
Department(s) Laboratory of Nematology
Laboratory of Entomology
Chair Soil Biology and Biological Soil Quality
PE&RC
EPS
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Biodiversity - High-throughput sequencing - Microscopy - Molecular approaches - Nematodes - QPCR - Soils
Abstract Below-ground nematodes are important for soil functioning, as they are ubiquitous and operate at various trophic levels in the soil food web. However, morphological nematode community analysis is time consuming and requires ample training. qPCR-based nematode identification techniques are well available, but high-throughput sequencing (HTS) might be more suitable for non-targeted nematode community analyses. We compared effectiveness of qPCR- and HTS-based approaches with morphological nematode identification while examining how climate warming-induced plant range expansion may influence below-ground nematode assemblages. We extracted nematodes from soil of Centaurea stoebe and C. jacea populations in Slovenia, where both plant species are native, and Germany, where C. stoebe is a range expander and C. jacea is native. Half of each nematode sample was identified morphologically and the other half was analysed using targeted qPCR and a novel HTS approach. HTS produced the highest taxonomic resolution of the nematode community. Nematode taxa abundances correlated between the methods. Therefore, especially relative HTS and relative morphological data revealed nearly identical ecological patterns. All methods showed lower numbers of plant-feeding nematodes in rhizosphere soils of C. stoebe compared to C. jacea. However, a profound difference was observed between absolute and relative abundance data; both sampling origin and plant species affected relative abundances of bacterivorous nematodes, whereas there was no effect on absolute abundances. Taken together, as HTS correlates with relative analyses of soil nematode communities, while providing highest taxonomic resolution and sample throughput, we propose a combination of HTS with microscopic counting to supplement important quantitative data on soil nematode communities. This provides the most cost-effective, in-depth methodology to study soil nematode community responses to changes in the environment. This methodology will also be applicable to nematode analyses in aquatic systems.
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