Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 538502
Title DNA assembly standards : Setting the low-level programming code for plant biotechnology
Author(s) Vazquez-Vilar, Marta; Orzaez, Diego; Patron, Nicola
Source Plant Science 273 (2018). - ISSN 0168-9452 - p. 33 - 41.
Department(s) Systems and Synthetic Biology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Biotechnology - CRISPR - DNA assembly - Genome engineering - Laboratory automation - Molecular cloning - Synthetic biology
Abstract Synthetic Biology is defined as the application of engineering principles to biology. It aims to increase the speed, ease and predictability with which desirable changes and novel traits can be conferred to living cells. The initial steps in this process aim to simplify the encoding of new instructions in DNA by establishing low-level programming languages for biology. Together with advances in the laboratory that allow multiple DNA molecules to be efficiently assembled together into a desired order in a single step, this approach has simplified the design and assembly of multigene constructs and has even facilitated the automated construction of synthetic chromosomes. These advances and technologies are now being applied to plants, for which there are a growing number of software and wetware tools for the design, construction and delivery of DNA molecules and for the engineering of endogenous genes. Here we review the efforts of the past decade that have established synthetic biology workflows and tools for plants and discuss the constraints and bottlenecks of this emerging field.
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