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Record number 538998
Title The positively-selected effector MiL648 of the root-knot nematode Meloidogyne incognita plays a role in tomato infections
Author(s) Verhoeven, Ava; Put, Sanne; Slootweg, E.J.; Raaij, D.R. van; Overmars, H.A.; Goverse, A.; Smant, G.
Event 3rd International Conference “Plant Biotic Stresses & Resistance Mechanisms", Vienna, 2018-07-02/2018-07-03
Department(s) Laboratory of Nematology
Publication type Poster (scientific)
Publication year 2018
Abstract Insight in the molecular basis of virulence can help us to identify new and more durable sources of resistance to the root-knot nematode Meloidogyne incognita in tomato. Recently, we identified the effector MiL648 by applying a genome-wide scan for positively selected secreted proteins. To acquire a better understanding of the function of MiL648, we are currently functionally characterising the protein in plants using host-induced gene silencing. Several in planta targets of the putative effector have now been found by a yeast-two-hybrid screening and interactions were checked using Co-IPs. To identify the role of the host targets in the infection process, homologs of the host targets were knocked out in Arabidopsis.
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