Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 539265
Title Vaccination of carp against SVCV with an oral DNA vaccine or an insect cells-based subunit vaccine
Author(s) Embregts, C.W.E.; Rigaudeau, D.; Tacchi, L.; Pijlman, G.P.; Kampers, L.; Veselý, T.; Pokorová, D.; Boudinot, P.; Wiegertjes, G.F.; Forlenza, M.
Source Fish and Shellfish Immunology (2018). - ISSN 1050-4648
DOI https://doi.org/10.1016/j.fsi.2018.03.028
Department(s) Cell Biology and Immunology
PE&RC
Laboratory of Virology
Systems and Synthetic Biology
WIAS
Aquaculture and Fisheries
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Alginate encapsulation - Baculovirus - DNA vaccine - Insect cells - SVCV glycoprotein
Abstract We recently reported on a successful vaccine for carp against SVCV based on the intramuscular injection of a DNA plasmid encoding the SVCV glycoprotein (SVCV-G). This shows that the intramuscular (i.m.) route of vaccination is suitable to trigger protective responses against SVCV, and that the SVCV G-protein is a suitable vaccine antigen. Yet, despite the general success of DNA vaccines, especially against fish rhabdoviruses, their practical implementation still faces legislative as well as consumer's acceptance concerns. Furthermore, the i.m. route of plasmid administration is not easily combined with most of the current vaccination regimes largely based on intraperitoneal or immersion vaccination. For this reason, in the current study we evaluated possible alternatives to a DNA-based i.m. injectable vaccine using the SVCV-G protein as the vaccine antigen. To this end, we tested two parallel approaches: the first based on the optimization of an alginate encapsulation method for oral delivery of DNA and protein antigens; the second based on the baculovirus recombinant expression of transmembrane SVCV-G protein in insect cells, administered as whole-cell subunit vaccine through the oral and injection route. In addition, in the case of the oral DNA vaccine, we also investigated the potential benefits of the mucosal adjuvants Escherichia coli lymphotoxin subunit B (LTB). Despite the use of various vaccine types, doses, regimes, and administration routes, no protection was observed, contrary to the full protection obtained with our reference i.m. DNA vaccine. The limited protection observed under the various conditions used in this study, the nature of the host, of the pathogen, the type of vaccine and encapsulation method, will therefore be discussed in details to provide an outlook for future vaccination strategies against SVCV.
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