Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 539515
Title Brucella pinnipedialis in grey seals (Halichoerus grypus) and harbor seals (Phoca vitulina) in the Netherlands
Author(s) Kroese, Michiel V.; Beckers, Lisa; Bisselink, Yvette J.W.M.; Brasseur, Sophie; Tulden, Peter W. van; Koene, Miriam G.J.; Roest, Hendrik I.J.; Ruuls, Robin C.; Backer, Jantien A.; Ijzer, Jooske; Giessen, Joke W.B. van der; Willemsen, Peter T.J.
Source Journal of Wildlife Diseases 54 (2018)3. - ISSN 0090-3558 - p. 439 - 449.
DOI https://doi.org/10.7589/2017-05-097
Department(s) CVI Diagnostics and Crisis
CVI Bacteriology and Epidemiology
IMARES Onderzoeksformatie
CVI Infection Biology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Brucella pinnipedialis - Halichoerus grypus - MALDI-TOF MS - Marine mammals - MLST - MLVA-16 - Phoca vitulina - The Netherlands
Abstract

Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals (Halichoerus grypus; n=11) and harbor seals (Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/ 39) seals were found to be positive for Brucella by IS711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.

Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.