Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 539666
Title Abscisic acid influences tillering by modulation of strigolactones in barley
Author(s) Wang, Hongwen; Chen, Wanxin; Eggert, Kai; Charnikhova, Tatsiana; Bouwmeester, Harro; Schweizer, Patrick; Hajirezaei, Mohammad R.; Seiler, Christiane; Sreenivasulu, Nese; Wirén, Nicolaus von; Kuhlmann, Markus
Source Journal of Experimental Botany 69 (2018)16. - ISSN 0022-0957 - p. 3883 - 3898.
DOI https://doi.org/10.1093/jxb/ery200
Department(s) Laboratory of Plant Physiology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Abscisic acid - barley - cereals - hormone regulation - phytohormone cross-talk - shoot branching - strigolactone biosynthesis - tillering
Abstract

Strigolactones (SLs) represent a class of plant hormones that are involved in inhibiting shoot branching and in promoting abiotic stress responses. There is evidence that the biosynthetic pathways of SLs and abscisic acid (ABA) are functionally connected. However, little is known about the mechanisms underlying the interaction of SLs and ABA, and the relevance of this interaction for shoot architecture. Based on sequence homology, four genes (HvD27, HvMAX1, HvCCD7, and HvCCD8) involved in SL biosynthesis were identified in barley and functionally verified by complementation of Arabidopsis mutants or by virus-induced gene silencing. To investigate the influence of ABA on SLs, two transgenic lines accumulating ABA as a result of RNAi-mediated down-regulation of HvABA 8'-hydroxylase 1 and 3 were employed. LC-MS/MS analysis confirmed higher ABA levels in root and stem base tissues in these transgenic lines. Both lines showed enhanced tiller formation and lower concentrations of 5-deoxystrigol in root exudates, which was detected for the first time as a naturally occurring SL in barley. Lower expression levels of HvD27, HvMAX1, HvCCD7, and HvCCD8 indicated that ABA suppresses SL biosynthesis, leading to enhanced tiller formation in barley.

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