|Title||Simple and effective purification approach to dissociate mixed water-insoluble α- and β-D-glucans and its application on the medicinal mushroom Fomitopsis betulina|
|Author(s)||Jesus, Liana Inara de; Smiderle, Fhernanda R.; Cordeiro, Lucimara M.C.; Freitas, Rilton A. de; Griensven, Leo J.L.D. van; Iacomini, Marcello|
|Source||Carbohydrate Polymers 200 (2018). - ISSN 0144-8617 - p. 353 - 360.|
|Department(s)||PRI BIOS Applied Metabolic Systems|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Fomitopsis betulina - NaOH treatment - Structural characterization - α- and β-D-glucans|
Differences in anomericity and in the branching degree of glucans lead to characteristic intermolecular association that influences their solubility in water or other solvents. A simple purification approach, based on the glucan solubility in aq. 0.1 M NaOH solution, was applied for the separation of mixed water-insoluble α-D-glucans from β-D-glucans extracted from fruiting bodies of Fomitopsis betulina, which is an underexploited medicinal mushroom. The results indicated that the β-D-glucan is constituted by (1→3)-linked β-D-Glcp units substituted at O-6 by non-reducing β-D-Glcp and (1→6)-linked β-D-Glcp units, while the α-D-glucan has a linear (1→3)-linked glucan structure. Thus, the 0.1 M NaOH treatment proved to be a simple, efficient and low-cost purification method for separation of water-insoluble glucans with different anomeric configurations and degree of branching that were interacting by intermolecular forces.