Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 539694
Title Simple and effective purification approach to dissociate mixed water-insoluble α- and β-D-glucans and its application on the medicinal mushroom Fomitopsis betulina
Author(s) Jesus, Liana Inara de; Smiderle, Fhernanda R.; Cordeiro, Lucimara M.C.; Freitas, Rilton A. de; Griensven, Leo J.L.D. van; Iacomini, Marcello
Source Carbohydrate Polymers 200 (2018). - ISSN 0144-8617 - p. 353 - 360.
DOI https://doi.org/10.1016/j.carbpol.2018.08.004
Department(s) PRI BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Fomitopsis betulina - NaOH treatment - Structural characterization - α- and β-D-glucans
Abstract

Differences in anomericity and in the branching degree of glucans lead to characteristic intermolecular association that influences their solubility in water or other solvents. A simple purification approach, based on the glucan solubility in aq. 0.1 M NaOH solution, was applied for the separation of mixed water-insoluble α-D-glucans from β-D-glucans extracted from fruiting bodies of Fomitopsis betulina, which is an underexploited medicinal mushroom. The results indicated that the β-D-glucan is constituted by (1→3)-linked β-D-Glcp units substituted at O-6 by non-reducing β-D-Glcp and (1→6)-linked β-D-Glcp units, while the α-D-glucan has a linear (1→3)-linked glucan structure. Thus, the 0.1 M NaOH treatment proved to be a simple, efficient and low-cost purification method for separation of water-insoluble glucans with different anomeric configurations and degree of branching that were interacting by intermolecular forces.

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