Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 539697
Title Facilitated delignification in CAD deficient transgenic poplar studied by confocal Raman spectroscopy imaging
Author(s) Segmehl, Jana S.; Keplinger, Tobias; Krasnobaev, Artem; Berg, John K.; Willa, Christoph; Burgert, Ingo
Source Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy 206 (2019). - ISSN 1386-1425 - p. 177 - 184.
DOI https://doi.org/10.1016/j.saa.2018.07.080
Department(s) Sub-department of Toxicology
RIKILT - Business unit Contaminants & Toxins
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) CAD deficient poplar - Cellulose conformational change - Facilitated delignification - Lignocellulosic biomass - Raman spectroscopy imaging - X-ray diffraction
Abstract

Lignocellulosic biomass represents the only renewable carbon resource which is available in sufficient amounts to be considered as an alternative for our fossil-based carbon economy. However, an efficient biochemical conversion of lignocellulosic feedstocks is hindered by the natural recalcitrance of the biomass as a result of a dense network of cellulose, hemicelluloses, and lignin. These polymeric interconnections make a pretreatment of the biomass necessary in order to enhance the susceptibility of the polysaccharides. Here, we report on a detailed analysis of the favourable influence of genetic engineering for two common delignification protocols for lignocellulosic biomass, namely acidic bleaching and soda pulping, on the example of CAD deficient poplar. The altered lignin structure of the transgenic poplar results in a significantly accelerated and more complete lignin removal at lower temperatures and shorter reaction times compared to wildtype poplar. To monitor the induced chemical and structural alterations at the tissue level, confocal Raman spectroscopy imaging, FT-IR spectroscopy, and X-ray diffraction were used.

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