Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 539846
Title Cell-type-specific promoter identification using enhancer trap lines
Author(s) Radoeva, Tatyana; Saiga, Shunsuke; Weijers, Dolf
Source In: Methods in Molecular Biology Humana Press Inc. (Methods in Molecular Biology ) - p. 127 - 139.
DOI https://doi.org/10.1007/978-1-4939-8657-6_8
Department(s) Biochemistry
EPS
Publication type Peer reviewed book chapter
Publication year 2018
Availibility Full text available from 2020-07-25
Keyword(s) Arabidopsis - Cell-type-specific promoters - Enhancer trap lines - Expression analysis - Insert location - TAIL-PCR
Abstract

Many developmental processes involve transitions between different cell identities as cells differentiate or undergo reprogramming. Cell identity specifications are generally associated with the activation and suppression of specific sets of genes mediated by transcription factors. Therefore, transcriptional reporters, such as promoters of cell-type-specific genes, are broadly used as cell identity markers in developmental biology. In Arabidopsis (Arabidopsis thaliana), a collection of GAL4/UAS enhancer trap lines is an established standard for inferring cell identity. However, only a few of these enhancer trap lines have been molecularly characterized, which limits their potential. Here, we describe an approach for a detailed characterization of expression and mapping of T-DNA insert location of GAL4/UAS enhancer trap lines. Additionally, we demonstrate how the acquired information can be further used for the generation of novel cell-type-specific promoters as well as for genotyping of enhancer trap lines.

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