Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 540639
Title Arabidopsis phospholipase C3 is involved in lateral root initiation and ABA responses in seed germination and stomatal closure
Author(s) Zhang, Qianqian; Wijk, Ringo van; Shahbaz, Muhammad; Roels, Wendy; Schooten, Bas Van; Vermeer, Joop E.M.; Zarza, Xavier; Guardia, Aisha; Scuffi, Denise; García-Mata, Carlos; Laha, Debabrata; Williams, Phoebe; Willems, Leo A.J.; Ligterink, Wilco; Hoffmann-Benning, Susanne; Gillaspy, Glenda; Schaaf, Gabriel; Haring, Michel A.; Laxalt, Ana M.; Munnik, Teun
Source Plant and Cell Physiology 59 (2018)3. - ISSN 0032-0781 - p. 469 - 486.
DOI https://doi.org/10.1093/pcp/pcx194
Department(s) Laboratory of Cell Biology
Laboratory of Plant Physiology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) ABA - Arabidopsis - Drought tolerance - Lateral root formation - Seed germination - Stomatal closure
Abstract

Phospholipase C (PLC) is well known for its role in animal signaling, where it generates the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), by hydrolyzing the minor phospholipid, phosphatidylinositol 4,5-bisphosphate (PIP2), upon receptor stimulation. In plants, PLC's role is still unclear, especially because the primary targets of both second messengers are lacking, i.e. the ligand-gated Ca2+ channel and protein kinase C, and because PIP2 levels are extremely low. Nonetheless, the Arabidopsis genome encodes nine PLCs. We used a reversed-genetic approach to explore PLC's function in Arabidopsis, and report here that PLC3 is required for proper root development, seed germination and stomatal opening. Two independent knock-down mutants, plc3-2 and plc3-3, were found to exhibit reduced lateral root densities by 10-20%. Mutant seeds germinated more slowly but were less sensitive to ABA to prevent germination. Guard cells of plc3 were also compromised in ABA-dependent stomatal closure. Promoter-b-glucuronidase (GUS) analyses confirmed PLC3 expression in guard cells and germinating seeds, and revealed that the majority is expressed in vascular tissue, most probably phloem companion cells, in roots, leaves and flowers. In vivo 32Pi labeling revealed that ABA stimulated the formation of PIP2 in germinating seeds and guard cell-enriched leaf peels, which was significantly reduced in plc3 mutants. Overexpression of PLC3 had no effect on root system architecture or seed germination, but increased the plant's tolerance to drought. Our results provide genetic evidence for PLC's involvement in plant development and ABA signaling, and confirm earlier observations that overexpression increases drought tolerance. Potential molecular mechanisms for the above observations are discussed.

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