|Title||Functional analysis of the HD-Zip transcription factor genes Oshox12 and Oshox14 in rice|
|Author(s)||Shao, Jingxia; Haider, Imran; Xiong, Lizhong; Zhu, Xiaoyi; Hussain, Rana Muhammad Fraz; Övernäs, Elin; Meijer, Annemarie H.; Zhang, Gaisheng; Wang, Mei; Bouwmeester, Harro J.; Ouwerkerk, Pieter B.F.|
|Source||PLoS One 13 (2018)7. - ISSN 1932-6203|
Laboratory of Plant Physiology
|Publication type||Refereed Article in a scientific journal|
The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice.