Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 541200
Title Applicability of the poultry qPCR method to detect DNA of poultry processed animal protein materials
Author(s) Scholtens, Ingrid M.J.; Prins, Theo W.; Margry, Rob J.C.F.; Dahlmans, Harald; Raamsdonk, Leo W.D. van
Source Food Control 96 (2019). - ISSN 0956-7135 - p. 53 - 58.
DOI https://doi.org/10.1016/j.foodcont.2018.08.035
Department(s) RIKILT - BU Toxicology Bioassays & Novel Foods
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Bovine spongiform encephalopathy (BSE) - Chicken - Duck - Feed - Geese - Poultry - Processed animal proteins (PAPs) - Sensitivity - Species-to-species ban - Transmissible spongiform encephalopathy (TSE) - Turkey
Abstract

After the Bovine Spongiform Encephalitis (BSE) crisis most processed animal proteins (PAPs) were banned from use in animal feed. For the foreseen reintroduction of pork PAPs in poultry feed, and poultry PAPs in pork feed and to comply with the species-to-species ban that prohibits cannibalism, a sensitive and specific TaqMan PCR detection method for poultry DNA has been designed and published. This poultry method is able to detect DNA of chicken, turkey, duck and geese in one PCR reaction. PAPs however, are a difficult and variable matrix. Therefore, the usability of the poultry method was investigated on a range of different poultry PAPs. It was shown that the poultry detection method is capable of detecting poultry DNA in eight out of nine different poultry PAPs mixed at a 0.1% level in chicken feed. The method can also detect at least 0.1% poultry PAPs mixed in pork PAPs. These results show that the poultry method fulfils the 0.1% detection limit requirement in the EU legislation.

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