Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 541253
Title Expression profile of Glossina pallidipes MicroRNAs during symptomatic and asymptomatic infection with Glossina pallidipes salivary gland hypertrophy virus (Hytrosavirus)
Author(s) Meki, Irene K.; Ince, Ikbal A.; Kariithi, Henry M.; Boucias, Drion G.; Ozcan, Orhan; Parker, Andrew G.; Vlak, Just M.; Oers, Monique M. van; Abd-Alla, Adly M.M.
Source Frontiers in Microbiology 9 (2018)SEP. - ISSN 1664-302X
DOI https://doi.org/10.3389/fmicb.2018.02037
Department(s) Laboratory of Virology
PE&RC
EPS
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Asymptomatic infection - Glossina - GpSGHV - Hytrosaviridae - Immune system - MiRNA - Sterile insect technique - Symptomatic
Abstract

The Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infects tsetse flies predominantly asymptomatically and occasionally symptomatically. Symptomatic infections are characterized by overt salivary gland hypertrophy (SGH) in mass reared tsetse flies, which causes reproductive dysfunctions and colony collapse, thus hindering tsetse control via sterile insect technique (SIT). Asymptomatic infections have no apparent cost to the fly's fitness. Here, small RNAs were sequenced and profiles in asymptomatically and symptomatically infected G. pallidipes flies determined. Thirty-eight host-encoded microRNAs (miRNAs) were present in both the asymptomatic and symptomatic fly profiles, while nine host miRNAs were expressed specifically in asymptomatic flies versus 10 in symptomatic flies. Of the shared 38 miRNAs, 15 were differentially expressed when comparing asymptomatic with symptomatic flies. The most up-regulated host miRNAs in symptomatic flies was predicted to target immune-related mRNAs of the host. Six GpSGHV-encoded miRNAs were identified, of which five of them were only in symptomatic flies. These virus-encoded miRNAs may not only target host immune genes but may also participate in viral immune evasion. This evidence of differential host miRNA profile in Glossina in symptomatic flies advances our understanding of the GpSGHV-Glossina interactions and provides potential new avenues, for instance by utilization of particular miRNA inhibitors or mimics to better manage GpSGHV infections in tsetse mass-rearing facilities, a prerequisite for successful SIT implementation.

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