|Title||Short lifespans of memory T-cells in bone marrow, blood, and lymph nodes suggest that T-cell memory is maintained by continuous self-renewal of recirculating cells|
|Author(s)||Baliu-Piqué, Mariona; Verheij, Myrddin W.; Drylewicz, Julia; Ravesloot, Lars; Boer, Rob J. de; Koets, Ad; Tesselaar, Kiki; Borghans, José A.M.|
|Source||Frontiers in Immunology 9 (2018)SEP. - ISSN 1664-3224|
CVI Bacteriology and Epidemiology
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Bone marrow - Deuterium - Lifespan - Lymphocyte turnover - Mathematical modeling - Memory T-cells - Stable isotope labeling|
Memory T-cells are essential to maintain long-term immunological memory. It is widely thought that the bone marrow (BM) plays an important role in the long-term maintenance of memory T-cells. There is controversy however on the longevity and recirculating kinetics of BM memory T-cells. While some have proposed that the BM is a reservoir for long-lived, non-circulating memory T-cells, it has also been suggested to be the preferential site for memory T-cell self-renewal. In this study, we used in vivo deuterium labeling in goats to simultaneously quantify the average turnover rates-and thereby expected lifespans-of memory T-cells from BM, blood and lymph nodes (LN). While the fraction of Ki-67 positive cells, a snapshot marker for recent cell division, was higher in memory T-cells from blood compared to BM and LN, in vivo deuterium labeling revealed no substantial differences in the expected lifespans of memory T-cells between these compartments. Our results support the view that the majority of memory T-cells in the BM are self-renewing as fast as those in the periphery, and are continuously recirculating between the blood, BM, and LN.