Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 541500
Title Growth and LC-PUFA production of the cold-adapted microalga Koliella antarctica in photobioreactors
Author(s) Suzuki, Hirono; Hulatt, Chris J.; Wijffels, René H.; Kiron, Viswanath
Source Journal of Applied Phycology (2018). - ISSN 0921-8971 - 17 p.
DOI https://doi.org/10.1007/s10811-018-1606-z
Department(s) VLAG
Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Cold-adapted microalgae - Eicosapentaenoic acid (EPA) - Flat-plate photobioreactor - LC-PUFA - Omega-3 fatty acid - Triacylglycerol
Abstract

Microalgae are excellent sources of polyunsaturated fatty acids (PUFAs), but only a few species have been thoroughly investigated in controlled photobioreactor conditions. In this work, the cold-adapted microalga Koliella antarctica (Trebouxiophyceae) was cultivated at 15 °C to optimize growth and PUFA production in bubble-tube and flat-plate photobioreactors. The impact of nitrogen starvation, phosphorus starvation, salinity, and light intensity on the growth, fatty acid, and protein content was investigated. After culture optimization, a maximum biomass productivity of 2.37 g L−1 day−1 and maximum cell density of 11.68 g L−1 were achieved. Among all conditions tested, the maximum total fatty acid (TFA) content measured 271.9 mg g−1 dry weight in the late stationary phase. Nitrogen and phosphorus starvation strongly induced neutral lipid (TAG) accumulation, up to 90.3% of TFA, which mostly consisted of the monounsaturated fatty acid C18:1n−9 (oleic acid, OA). PUFAs were also abundant and together accounted for 30.3–45.8% of total triacylglycerol (TAG). The highest eicosapentaenoic acid (EPA) content (C20:5n−3) amounted to 6.7 mg g−1 dry weight (4.9% TFA) in control treatments, while the highest arachidonic acid (ARA) content (C20:4n−6) was 9.6 mg g−1 dry weight (3.5% TFA) in the late stationary phase. Phosphorus starvation was an effective strategy to obtain high total fatty acid yields (mg L−1) while maintaining the protein, total PUFA, and omega-3 fatty acid contents.

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