Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 541516
Title Short communication: detection and molecular characterization of hepatitis E virus in domestic animals of São Tomé and Príncipe
Author(s) Mesquita, João Rodrigo; Istrate, Claudia; Santos-Ferreira, Nânci L.; Ferreira, Ana S.; Abreu-Silva, Joana; Veiga, José; Poel, Wim H.M. van der; Nascimento, Maria S.J.
Source Tropical Animal Health and Production (2018). - ISSN 0049-4747
DOI https://doi.org/10.1007/s11250-018-1700-x
Department(s) CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Domestic animals - Hepatitis E virus - São Tomé e Príncipe - Zoonoses
Abstract

As in most of the African continent, the status of hepatitis E virus (HEV) infection in domestic animals in São Tomé and Príncipe, an archipelago off the western equatorial coast of Central Africa, is also completely unknown. In the present study, we investigated the presence of HEV among domestic animals in São Tomé and Príncipe. A total of 93 stool samples from different animal species (goat, cow, pig, chicken, duck, and monkey) were tested for HEV RNA using two real-time RT-PCR assays, followed by a nested RT-PCR assay for sequencing and phylogenetic analysis. A total of six samples (1 cow stool and 5 pig stools) were found to be positive for HEV RNA of which one pig stool was positive by broad spectrum nested RT-PCR. Phylogenetic analysis showed that the retrieved sequence clustered within HEV subgenotype 3f, similar to zoonotic strains of European countries and posing interesting questions on past introduction of European HEV into São Tomé and Príncipe archipelago. This is the first report describing the presence and molecular characterization of HEV in São Tomé and Príncipe.

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