Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 541521
Title Tracking disease resistance deployment in potato breeding by enrichment sequencing
Author(s) Armstrong, Miles R.; Vossen, Jack; Lim, Tze Yin; Hutten, Ronald C.B.; Xu, Jianfei; Strachan, Shona M.; Harrower, Brian; Champouret, Nicolas; Gilroy, Eleanor M.; Hein, Ingo
Source Plant Biotechnology Journal (2018). - ISSN 1467-7644
DOI https://doi.org/10.1111/pbi.12997
Department(s) WUR PB Resistance in Solanaceae
PBR Biodiversiteit en Genetische Variatie
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) breeding - crops - disease resistance genes - dRenSeq - potato - tracking of NLRs
Abstract

Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans) gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.

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