Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 541559
Title Metabolic modelling and energy parameter estimation of Tetradesmus obliquus
Author(s) León-Saiki, G.M.; Ferrer Ledo, Narcís; Lao-Martil, David; Veen, Douwe van der; Wijffels, René H.; Martens, Dirk E.
Source Algal Research 35 (2018). - ISSN 2211-9264 - p. 378 - 387.
DOI https://doi.org/10.1016/j.algal.2018.09.008
Department(s) Bioprocess Engineering
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Compartmentalized metabolism - Flux Balance Analysis (FBA) - Maintenance requirement - Microalgae - Scenedesmus obliquus
Abstract

We developed a metabolic network describing the primary metabolism of Tetradesmus obliquus aimed to get a better understanding of metabolism to improve industrial production. The network includes 351 reactions with 183 metabolites distributed over 4 compartments: cytosol, chloroplast, mitochondria, and extracellular space. The energy requirements for biomass assembly and maintenance (Kx and mATP, respectively) were experimentally determined from batch cultures and included in the model. The determined values were 121.02 mmolATP·gDW −1 for Kx and 0.66 mmolATP·gDW −1·h−1 for the mATP. The maintenance value found for T. obliquus is, to our knowledge, one of the lowest reported in literature for microalgae. This low value is also in agreement with the photon maintenance requirement found experimentally for T. obliquus (1.18 mmolph·gDW −1·h−1). Finally, the theoretical maximum yields based on the model for biomass, triacylglycerides (TAG), and starch yield on light were calculated to be 1.15 g·molph −1, 1.05 gTAG·molph −1, and 2.69 gstarch·molph −1.

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