Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 541623
Title Optimisation of coding sequence for functional protein expression
Author(s) Westerhof, L.B.; Bakker, J.
Source Octrooinummer: WO2016086988, verleend: 2016-06-09.
Department(s) EPS
Laboratory of Nematology
Publication type Patent
Publication year 2016
Abstract The present invention relates to an approach aimed at the modification of codons in individual polynucleotide sequences encoding a heterologous protein of interest, without altering the amino acid sequence of the polypeptide to enhance the amount of functional expression in a host organism of interest. In its broadest aspect, this approach exploits redundancy in the genetic code by providing a universal set of codons which may be used at certain positions in the polynucleotide sequence in order to achieve improved heterologous protein production in a range of host cells. The present invention also relates to specific codons which may be used to increase protein expression in particular hosts. The present invention also relates to the optimization of the translation efficiency of messenger RNAs on the basis of their secondary structure characteristics, and the provided set of criteria may be used to increase protein expression in particular hosts.
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