|Title||Process for producing itaconic acid under anaerobic conditions|
|Author(s)||Weusthuis, R.A.; Vuoristo, K.S.; Eggink, G.; Sanders, J.P.M.|
|Source||Octrooinummer: WO2016131818, verleend: 2016-08-25.|
|Abstract||The present invention relates to a method for the production of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid in a suitable fermentation medium, thereby to produce itaconic acid, wherein: (a) the recombinant cell: (i) overexpresses cis-aconitate decarboxylase; and (ii) overexpresses a part of the citric acid cycle and/or has reduced activity of a native metabolic route to acetate and/or lactate; and, optionally, (b) the fermentation is carried out under anaerobic conditions.