Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 542586
Title Comparison of cryopreservation techniques for cells of the marine sponge dysidea Etheria
Author(s) Munroe, Stephanie; Martens, Dirk E.; Sipkema, Detmer; Pomponi, Shirley A.
Source Cryo-Letters 39 (2018)4. - ISSN 0143-2044 - p. 269 - 278.
Department(s) Bioprocess Engineering
VLAG
WIMEK
Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Cryopreservation - Cryoprotectants - DMSO - Glycerol - Porifera
Abstract

BACKGROUND: Cryopreservation is a commonly used method for the long-term storage of cell lines and provides a stable source of cells for experiments, allowing researchers to study species that are not geographically nearby, and useful to progress studies on sponge cell biotechnology. OBJECTIVE: The marine sponge Dysidea etheria was chosen as our model organism to evaluate the impact and effectiveness of two commonly used cryoprotectants, dimethyl sulfoxide (DMSO) and glycerol. MATERIALS AND METHODS: By testing a range of concentrations (3-10% DMSO, 10-50% glycerol), we determined the optimal cryoprotectant for D. etheria based on its ability to preserve viable cells and optimize recovery after cryopreservation. RESULTS: Cells cryopreserved in DMSO had significantly higher viability after cryopreservation than those cryopreserved in glycerol. Cells cryopreserved in glycerol had irregular morphology as well as lower recovery of viable cells than those from DMSO treatments. CONCLUSION: Our results demonstrate that the optimal cryoprotectant for sponge cells, without a significant loss of viability, is 5-8% DMSO. This approach can be used to optimize cryopreservation methods for cells of other marine invertebrate species.

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