Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 543528
Title Prolonged intake of hyperproteic casein-based diet promotes a molecular environment leading to liver triacylglycerol deposition and increases markers of hepatic damage in rats
Author(s) Schothorst, E.M. van; Keijer, J.; Diaz, R.; Palou, A.; Oliver, P.
Event 25th European Congress on Obesity, Vienna, 2018-05-23/2018-05-26
Department(s) WIAS
Human and Animal Physiology
Publication type Abstract in scientific journal or proceedings
Publication year 2018
Abstract Introduction: High protein (HP) diets have been associated to body weight loss and positive metabolic effects on obese subjects. However, controversy exists on the effects of long-term intake of these diets, as more recent reports point to health risk and higher mortality. Liver is a key organ involved in macronutrient handling, thus, we aimed to analyse the effects of HP diets on liver metabolism and health.
Methods: We performed a transcriptome analysis on liver of healthy adult male Wistar rats fed for 4 months with a casein-rich HP diet and analysed adiposity and molecular parameters related to metabolic syndrome and liver injury.
Results: Compared to rats on a control diet, HP-fed animals, that ingested 2.3 times higher amount of protein than controls, showed a lower cumulative food intake and lower body weight; although this lower body weight was not related to decreased adiposity. HP-fed animals presented lower serum cholesterol levels and were apparently healthy according to parameters related to metabolic syndrome: no differences were found in circulating non-esterified fatty acids or triaclyglicerols (TG) in comparison to controls. In liver, long-term intake of the casein-rich diet had an impact on metabolic pathways related with amino acid uptake/metabolism and lipid synthesis, indicative of higher TG deposition. Liver transcriptomic analysis also revealed up-regulation of immune-related genes and changes in expression of genes involved in acid-base maintenance and oxidative stress, pointing to alterations in the pH balance due to the high acid load of the diet, which has been linked to liver/health damage. In line with these transcriptomic changes, clear functional signs of unhealthy effects, such increased liver TG content and increased serum markers of hepatic injury/inflammation (aspartate transaminase, C-reactive protein and TNF-alpha) were observed. Moreover, chronic intake of the HP diet produced a dramatic increase of hepatic HSP90, a marker of liver injury.
Conclusion: A drastic and prolonged increase in diet protein intake, resulting in a high acid load, induces a hepatic transcriptome signature reflecting increased TG deposition and increased levels of markers of liver/health injury.
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