Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 545303
Title Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)
Author(s) Bogdanovic, Milica; Cankar, K.; Todorovic, Sladana; Dragicevic, Milan; Simonovic, Ana; Houwelingen, A.M.M.L. van; Schijlen, E.G.W.M.; Schipper, A.; Gagneul, David; Hendriks, Theo; Quillet, Marie-Christine; Bouwmeester, H.J.; Bosch, H.J.; Beekwilder, M.J.
Source Industrial Crops and Products 129 (2019). - ISSN 0926-6690 - p. 253 - 260.
Department(s) PRI BIOS Applied Metabolic Systems
Laboratory of Plant Physiology
PRI BIOS Applied Bioinformatics
Publication type Refereed Article in a scientific journal
Publication year 2019
Availibility Full text available from 2021-03-01
Abstract Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis of
leaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.