Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 60131
Title Molecular evidence that Meloidogyne hapla, M. Chitwoodi and M. Fallax are distinct biological entities
Author(s) Beek, J.G. van der; Folkertsma, R.; Poley, L.M.; Koert, P.H.G. van; Bakker, J.
Source Fundamental and applied nematology 20 (1997)5. - ISSN 1164-5571 - p. 513 - 520.
Department(s) Laboratory of Nematology
Publication type Refereed Article in a scientific journal
Publication year 1997
Abstract Six isolates of Meloidogyne hapla, including four race A and two race B isolates, eight isolates of M. chitwoodi, and five isolates of M. fallax were submitted to two-dimensional gel electrophoresis (2-DGE) to study the similarity between the various isolates of the three species based upon total soluble proteins. For each isolate, two independent samples of 50 young egg-producing females were extracted from roots. Each sample was used to produce one mini 2-D gel. Mini 2-DGE showed an average of 400 protein spots per gel. Within each species, every gel was compared to all the others to identify two types of polymorphic spots: isoelectric point (IP) variants and present-absent (PA) variants. Thirteen PA and nine IP variants were observed among M. hapla isolates, eight PA and no IP variants among M. chitwoodi isolates, and two PA and no IP variants among M. fallax isolates, which represents 5.0, 2.2, and 0.6 % polymorphisms among isolates of the three respective species. These percentages imply small intraspecific variation within each of these species. They also confirm these species to be clearly delineated biological groups, as similarity between these species is significantly lower than similarity between isolates within each species (Van der Beek et al., unpubl.). Using UPGMA, the nine IP variants of M. hapla resulted in a similarity dendrogram, which clearly separated the race A isolates from the race B isolates.
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