Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 61360
Title Prospects for introgressing tomato chromosomes into the potato genome: An assessment through GISH analysis
Author(s) Garriga Calderé, F.; Huigen, D.J.; Jacobsen, E.; Ramanna, M.S.
Source Genome 42 (1999)2. - ISSN 0831-2796 - p. 282 - 288.
DOI https://doi.org/10.1139/gen-42-2-282
Department(s) Laboratory of Plant Breeding
EPS
Publication type Refereed Article in a scientific journal
Publication year 1999
Abstract With a view to assess the possibility of homoeologous pairing and crossing-over between the chromosomes of potato (Solanum tuberosum) and tomato (Lycopersicon esculentum), a somatic fusion hybrid and two monosomic alien tomato addition genotypes were investigated through genomic in situ hybridisation (GISH). The somatic fusion hybrid, C31-17-51, was near hexaploid (2n = 6x - 4 = 68) possessing 46 potato chromosomes + 20 tomato chromosomes + 2 translocated chromosomes. The two alien addition genotypes were near tetraploids (2n = 4x + 1 = 49) and consisted of monosomic alien additions for tomato chromosome 1 in genotype 2103-1, and tomato chromosome 8 in genotype 2301-2. In the fusion hybrid the tomato pachytene chromosome identification revealed that the chromosomes 1, 2, 5, 6, 7, 10, and 12 were in diploid condition whereas among those that were in haploid condition, three could be identified viz., 4, 9, and 11. The remaining three chromosomes could not be cytologically identified. Although the chromosomes with translocated segments could not be identified at the pachytene stage due to technical difficulties, there was clear evidence for the presence of a reciprocal translocation observed at diakinesis and metaphase I stages. Because of autosyndetic pairing of the translocated segments, it gave a false impression as if there was a high frequency (86.0%) of allosyndetic pairing. In contrast to the fusion hybrid, the two alien monosomic addition genotypes showed a very low frequency of allosyndetic pairing, namely 1.1 and 1.3% respectively for the monosomic additions 1 and 8. In the genotype 2301-2, monosomic addition for tomato chromosome 8, crossing-over between the homoeologous chromosomes was estimated to occur in 0.8% of the meiotic cells investigated. Despite this low frequency of homoeologous pairing and crossing-over, there is a possibility for introgressing tomato chromosomal DNA into the potato genome through intergenomic recombination.
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