Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 61492
Title Differential substrate behaviour of phenol and aniline derivatives during conversion by horseradish peroxidase
Author(s) Haandel, M.J.H. van; Claassens, M.M.J.; Hout, N. van der; Boersma, M.G.; Vervoort, J.; Rietjens, I.M.C.M.
Source Biochimica et biophysica acta-protein structure and molecular enzymology 1435 (1999). - ISSN 0167-4838 - p. 22 - 29.
Department(s) Biochemistry
Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 1999
Abstract For the first time saturating overall kcat values for horseradish peroxidase (HRP) catalysed conversion of phenols and anilines are described. These kcat values correlate quantitatively with calculated ionisation potentials of the substrates. The correlations for the phenols are shifted to higher kcat values at similar ionisation potentials as compared to those for anilines. 1H-NMR T1 relaxation studies, using 3-methylphenol and 3-methylaniline as the model substrates, revealed smaller average distances of the phenol than of the aniline protons to the paramagnetic Fe3 centre in HRP. This observation, together with a possibly higher extent of deprotonation of the phenols than of the anilines upon binding to the active site of HRP, may contribute to the relatively higher HRP catalysed conversion rates of phenols than of anilines.
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