Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 63162
Title Bioavailability of genistein, daidzein, and their glycosides in intestinal epithelial Caco-2 cells
Author(s) Steensma, A.; Noteborn, H.P.J.M.; Jagt, R.C.M. van der; Polman, Th.H.G.; Mengelers, M.J.B.; Kuiper, H.A.
Source Environmental Toxicology and Pharmacology 7 (1999). - ISSN 1382-6689 - p. 209 - 212.
Department(s) RIKILT
Sub-department of Toxicology
Publication type Refereed Article in a scientific journal
Publication year 1999
Abstract In this study information was obtained on bioavailability of genistein, daidzein and their glycosides in human intestinal epithelial Caco-2 cells grown on semi-permeable filters. The integrity of Caco-2 monolayers was confirmed by transepithelial electrical resistance measurements and by determination of the permeability of the radioactive marker polyethylene glycol (PEG4000). After 6 h approximately 30–40% of genistein and daidzein added at the apical side was transported to the basolateral side and this level was maintained for 24 h, The glycosides were barely transported through the Caco-2 cells. No significant metabolism of genistein and daidzein in the Caco-2 cells occurred, whereas the glycosides were mainly metabolised to their respective aglycones. Obviously, our data indicates that Caco-2 cells contain an endogenous glycosidase activity.
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