Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 63355
Title Isolation and physical characterization of an exocellular polysaccharide
Author(s) Tuinier, R.; Zoon, P.; Olieman, C.; Cohen Stuart, M.A.; Fleer, G.J.; Kruif, C.G. de
Source Biopolymers 49 (1999). - ISSN 0006-3525 - p. 1 - 9.
DOI http://dx.doi.org/10.1002/(SICI)1097-0282(199901)49:1<1::AID-BIP1>3.0.CO;2-B
Department(s) Physical Chemistry and Colloid Science
VLAG
Publication type Refereed Article in a scientific journal
Publication year 1999
Abstract The physical properties of a polysaccharide produced by the lactic acid bacterium Lactococcus lactis subsp. cremoris strain NIZO B40 were investigated. Separation of the polysaccharide from most low molar mass compounds in the culture broth was performed by filtration processes. Residual proteins and peptides were removed by washing with a mixture of formic acid, ethanol, and water. Gel permeation chromatography (GPC) was used to size fractionate the polysaccharide. Fractions were analyzed by multiangle static light scattering in aqueous 0.10M NaNO3 solutions from which a number- (Mn) and weight-averaged (Mw) molar mass of (1.47 ± 0.06) ? 103 and (1.62 ± 0.07) ? 103 kg/mol, respectively, were calculated so that Mw/Mn 1.13. The number-averaged radius of gyration was found to be 86 ± 2 nm. From dynamic light scattering an apparent z-averaged diffusion coefficient was obtained. Upon correcting for the contributions from intramolecular modes by extrapolating to zero wave vector a hydrodynamic radius of 86 ± 4 nm was calculated. Theoretical models for random coil polymers show that this z-averaged hydrodynamic radius is consistent with the z-averaged radius of gyration, 97 ± 3 nm, as found with GPC.
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