Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 64192
Title Outward component of photoinduced current in chloroplasts of Peperomia metallica
Author(s) Cherkashin, A.A.; Bulychev, A.A.; Vredenberg, W.J.
Source Biologicheskie Membrany 17 (2000). - ISSN 0233-4755 - p. 377 - 386.
Department(s) Laboratory of Plant Physiology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract The photoinduced currents in whole chloroplasts of Peperomia metallica were studied using suction electrodes and single-turnover flashes. The kinetic profile of the photocurrent contained a minor outward component (rise time, 100 micros). Local application (from the inside of the pipette) of a photosystem 2 inhibitor, DCMU, rapidly suppressed the outward current; conversely, addition of DCMU to the outer medium produced a transient stimulation of the outward component. Permeabilization of the tip-located membrane fragments with Triton X-100 eliminated the outward current, but had no significant influence on the inward current. The data suggest that the outward current originated in the tip-located nonruptured portions of the thylakoid membrane. Different involvement of two photosystems in the generation of the outward current indicates that granal thylakoids enriched with photosystem 2 are less susceptible to the rupture in the pipette tip as compared with stromal thylakoids.
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