Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 64267
Title Antisense suppression of a potato alpha-SNAP homologue leads to alterations in cellular development and assimilate distribution
Author(s) Bachem, C.W.B.; Oomen, R.J.F.J.; Kuyt, S.; Horvath, B.M.; Claassens, M.M.J.; Vreugdenhil, D.; Visser, R.G.F.
Source Plant Molecular Biology 43 (2000)4. - ISSN 0167-4412 - p. 473 - 482.
Department(s) Laboratory of Plant Breeding
Laboratory of Plant Physiology
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract Using the cDNA-AFLP method, we have isolated a transcript-derived fragment (TDF) which shows a differential expression pattern during tuber organogenesis of Solanum tuberosum L. The TDF was used to isolate a cDNA clone carrying a 1.5 kb insert and potentially coding for a 32.5 kDa peptide which, by homology, represents a potato homologue of an alpha-snap gene and has been designated Stsnap. Northern analysis showed that the Stsnap gene is expressed in actively dividing tissues throughout the potato plant. Analysis of genomic DNA from potato revealed that the Stsnap gene is likely to be a single-copy gene. The expression of antisense Stsnap cDNA under the control of the CaMV 35S promoter results in plants with an altered morphology such as curled leaves. Several of these transgenic lines also display cellular and developmental abnormalities with distinct changes in assimilate transport including accumulation of starch and soluble sugars in source leaves. We argue that these findings are consistent with the hypothetical function of the StSNAP gene product in vesicle targeting and fusion during plant development.
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