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Record number 64970
Title Two additive QTLs conferring broad-spectrum resistance in potato to Globodera pallida are localized on resistance gene clusters
Author(s) Rouppe van der Voort, J.; Vossen, E. van der; Bakker, E.; Overmars, H.; Zandvoort, P. van; Hutten, R.; Klein Lankhorst, R.; Bakker, J.
Source Theoretical and Applied Genetics 101 (2000). - ISSN 0040-5752 - p. 1122 - 1130.
Department(s) Laboratory of Nematology
Laboratory of Plant Breeding
PRI Biodiversity and Breeding
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract Broad-spectrum resistance in potato to the potato cyst nematode (PCN) is commonly regarded as a complex inherited trait. Yet, in this paper we show that, by use of a selected set of PCN test populations, broad-spectrum resistance to the species Globodera pallida can be fully ascribed to the action of two loci: Gpa5 and Gpa6. These loci were readily mapped by means of a strategy based on two steps. Firstly, the chromosomal localization of both loci was assessed by use of an online catalogue of AFLP markers covering a substantial part of the potato genome ( Subsequently the chromosomal regions of both loci were identified by means of CAPS markers based on RFLP insert sequences. Locus Gpa5 explains at least 61% of the genetic variation. This locus maps to chromosome 5 on a region which has previously been shown to harbor resistance factors to viral (Nb, Rx2), fungal (R1) and nematodal (Gpa, Grp1) pathogens. The Gpa6 locus exhibits a minor effect on the resistance (24%) and acts additively to Gpa5. Interestingly, the Gpa6 locus maps to a region on chromosome 9 where, in the homoeologous tomato genome, the virus resistance gene Sw-5 resides as part of a resistance gene cluster. In potato, resistance to potato virus X has been reported in the vicinity of this region. The map location of Gpa6 indicates the presence of a resistance gene cluster at the end of the long arm of chromosome 9 of potato.
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