Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 66368
Title Adaptation of the food-borne pathogen Bacillus cereus to carvacrol
Author(s) Ultee, A.; Kets, E.P.W.; Alberda, M.; Hoekstra, F.A.; Smid, E.J.
Source Archives of Microbiology 174 (2000). - ISSN 0302-8933 - p. 233 - 238.
DOI http://dx.doi.org/10.1007/s002030000199
Department(s) Agrotechnological Research Institute
Laboratory of Plant Physiology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract Carvacrol, a natural antimicrobial compound present in the essential oil fraction of oregano and thyme, is bactericidal towards Bacillus cereus. A decrease of the sensitivity of B. cereus towards carvacrol was observed after growth in the presence of non-lethal carvacrol concentrations. A decrease of the melting temperature (Tm) of membranes from 20.5 °C to 12.6 °C was the immediate effect of the addition of carvacrol. Cells adapted to 0.4 mM carvacrol showed a lower membrane fluidity than non-adapted cells. Adaptation of 0.4 mM carvacrol increased the Tm from 20.5 °C to 28.3 °C. The addition of carvacrol to cell suspensions of adapted B. cereus cells decreased Tm again to 19.5 °C, approximately the same value as for the non-adapted cells in the absence of carvacrol. During adaptation, changes in the fatty acid composition were observed. The relative amount of iso-C13:0, C14:0 and iso-C15:0 increased and cis-C16:1 and C18:0 decreased. The head-group composition also changed, two additional phospholipids were formed and one phospholipid was lacking in the adapted cells. It could be concluded that B. cereus adapts to carvacrol when present at non-lethal concentrations in the growth medium by lowering its membrane fluidity by changing the fatty acid and head-group composition
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