Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 66377
Title Esterification and glycosydation of oligogalacturonides : examination of the reaction products using MALDI-TOF MS and HPAEC
Author(s) Alebeek, G.J.W.M. van; Zabotina, O.; Beldman, G.; Schols, H.A.; Voragen, A.G.J.
Source Carbohydrate Polymers 43 (2000). - ISSN 0144-8617 - p. 39 - 46.
Department(s) Food Chemistry Group
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract Methyl-esterified and methyl-glycosydated oligogalacturonides (oligoGalA) were produced to be used as substrates for the characterization of pectinolytic enzymes acting on the homogalacturonan backbone. The reactions were monitored with recently developed techniques like high-performance anion-exchange chromatography (HPAEC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) that allow sensitive monitoring of the reactions. MALDI-TOF MS reveals the degree of esterification and or glycosydation. HPAEC at neutral pH separates not only oligogalacturonides with a different degree of esterifcation but seems to separate some isomers of e.g. monomethyl- and dimethyl-triGalA as well. Breakdown products formed by hydrolysis side reactions were revealed and could even be quantified by HPAEC pH 12 analysis. Using these techniques the conditions for each of the reactions were optimized. Esterification was performed best at concentrations of maximal 0.02N sulfuric acid in anhydrous methanol at 4oC. Hardly any glycosydation occurs and the level of hydrolysis of the oligoGalA was less than 5%. Methyl-glycosydation and simultaneous esterification was performed best in 0.1N sulfuric acid in anhydrous methanol at room temperature. HPAEC revealed only a limited hydrolysis (<11%).
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