Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 66890
Title Cloning and characterization of an epoxide hydrolase-encoding gene from Rhodotorula glutinis
Author(s) Visser, H.; Vreugdenhil, S.; Bont, J.A.M. de; Verdoes, J.C.
Source Applied Microbiology and Biotechnology 53 (2000)4. - ISSN 0175-7598 - p. 415 - 419.
Department(s) Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract We cloned and characterized the epoxide hydrolase gene, EPH1, from Rhodotorula glutinis. The EPH1 open reading frame of 1230 bp was interrupted by nine introns and encoded a polypeptide of 409 amino acids with a calculated molecular mass of 46.3 kDa. The amino acid sequence was similar to that of microsomal epoxide hydrolase, which suggests that the epoxide hydrolase of R. glutinis also belongs to the alpha/beta hydrolase fold family. EPH1 cDNA was expressed in Escherichia coli and resting cells showed a specific activity of 200 nmol min(-1)(mg protein)(-1) towards 1,2-epoxyhexane.
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