Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 67005
Title Effects of ageing on the in vitro fermentation of cell walls and cell contents of entire, fractioned and composite leaves of Italian ryegrass
Author(s) Williams, B.A.; Oostdam, A.J.; Groot, J.C.J.; Boer, H.; Tamminga, S.
Source Journal of the Science of Food and Agriculture 80 (2000). - ISSN 0022-5142 - p. 484 - 490.
Department(s) Animal Nutrition
Publication type Refereed Article in a scientific journal
Publication year 2000
Abstract Changes in fermentability of cell walls and cell contents of Italian ryegrass leaves at two stages of maturity were measured to explain the generally observed decline in herbage quality with ageing. A herbage fractionation method was developed to separate cell contents and cell walls. Cell walls were either untreated or protease-treated. Fermentation characteristics of all cell wall and cell contents fractions, as well as whole and recombined leaves, were measured using cumulative gas and volatile fatty acid (VFA) production. The gas production profiles of all substrates contained two significant phases. After fractionation, addition of the profiles from separated cell contents and untreated cell walls resulted in the same profile as for the recombined leaf. The strongest reduction in gas and VFA production due to leaf ageing was observed for cell contents. The increased ratio between branched and straight chain VFAs suggested that there had been an increase in the protein fermentation. Treatment of cell walls may have removed some easily fermentable cell wall components, as seen in the small differences in gas and VFA production between whole and recombined leaves. It has been concluded that the phases of gas production, separable in kinetic fermentability studies of complex animal feeds, need to be interpreted with caution. The large reduction in fermentability of cell contents with plant maturity, compared with the cell walls, indicated the importance of determining the role of cell contents in herbage quality studies, as the cell contents clearly do not remain uniform.
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