- J. Keijer (1)
- E.H.M. Kramer (1)
- R. Meer van der (1)
- T.W. Prins (2)
- M.A.A. Schepens (1)
- A.J. Schonewille (1)
- E.M. Schothorst van (1)
- P. Stam (2)
- C. Vink (1)
- P.B. Visser (2)
- Z. Yan (2)
Supplemental calcium attenuates the Colitis-Related increase in Diarrhea, intestinal Permeability, and Extracellular Matrix Breakdown in HLA-B27 Transgenic Rats
Schepens, M.A.A. ; Schonewille, A.J. ; Vink, C. ; Schothorst, E.M. van; Kramer, E.H.M. ; Hendriks, T. ; Brummer, R.J. ; Keijer, J. ; Meer, R. van der; Bovee - Oudenhoven, I.M.J. - \ 2009
The Journal of Nutrition 139 (2009)8. - ISSN 0022-3166 - p. 1525 - 1533.
inflammatory-bowel-disease - dietary calcium - bile-acids - gene-expression - crohns-disease - fatty-acids - ulcerative-colitis - transgenic rats - salmonella - colon
We have shown in several controlled rat and human infection studies that dietary calcium improves intestinal resistance and strengthens the mucosal barrier. Reinforcement of gut barrier function may alleviate inflammatory bowel disease (IBD). Therefore, we investigated the effect of supplemental calcium on spontaneous colitis development in an experimental rat model of IBD. HLA-B27 transgenic rats were fed a purified high-fat diet containing either a low or high calcium concentration (30 and 120 mmol CaHPO4/kg diet, respectively) for almost 7 wk. Inert chromium EDTA (CrEDTA) was added to the diets to quantify intestinal permeability by measuring urinary CrEDTA excretion. Relative fecal wet weight was determined to quantify diarrhea. Colonic inflammation was determined histologically and by measuring mucosal interleukin (IL)-1ß. In addition, colonic mucosal gene expression of individual rats was analyzed using whole-genome microarrays. The calcium diet significantly inhibited the increase in intestinal permeability and diarrhea with time in HLA-B27 rats developing colitis compared with the control transgenic rats. Mucosal IL-1ß levels were lower in calcium-fed rats and histological colitis scores tended to be lower (P = 0.08). Supplemental calcium prevented the colitis-induced increase in the expression of extracellular matrix remodeling genes (e.g. matrix metalloproteinases, procollagens, and fibronectin), which was confirmed by quantitative real-time PCR and gelatin zymography. In conclusion, dietary calcium ameliorates several important aspects of colitis severity in HLA-B27 transgenic rats. Reduction of mucosal irritation by luminal components might be part of the mechanism. These results show promise for supplemental calcium as effective adjunct therapy for IBD
QTL analysis of variation for vigour in rose
Yan, Z. ; Visser, P.B. ; Hendriks, T. ; Prins, T.W. ; Stam, P. ; Dolstra, O. - \ 2007
Euphytica 154 (2007)1-2. - ISSN 0014-2336 - p. 53 - 62.
genetic-linkage maps - quantitative trait loci - aflp markers - construction - rflp - rapd
The improvement of energy efficiency in the greenhouse production of cut rose and pot rose can be achieved through the use of rose cultivars having vigorous growth. A better understanding of the inheritance of vigour and its related traits will assist the breeding activities. Quantitative trait locus (QTL) analyses were performed with the help of an integrated linkage map of a diploid rose population originating from a cross between Rosa multiflora-derived genotypes. The underlying datasets for ten vigour-related traits were collected in an evaluation study of this population in two greenhouse experiments with suboptimal temperatures for growth. We identified ten chromosomal regions, scattered over the seven linkage groups, containing QTLs for these traits. Considering each trait separately, we detected a total of 42 QTLs. Among these QTLs, 24 were found in both of the experiments, eight and ten were specific to either of the two experiments. The number of QTLs for individual traits varied from three to five with a respective contribution to the phenotypic variation from 12 to 35%. QTLs for highly correlated traits frequently co-localized, indicating a common genetic basis. Clustering of QTLs for different traits was noted in some chromosome regions, for instance, one on chromosome 2 included major QTLs for eight of ten traits under study, suggesting co-localization of several separate genes or/and the occurrence of various genes having pleiotropic effects. The discovery of markers associated to QTL regions is in roses the first step towards marker-assisted selection for vigour improvement enabling the transfer of useful QTL-alleles of R. multiflora to pot and cut roses
Vigour evaluation for genetics and breeding in rose
Yan, Z. ; Dolstra, O. ; Hendriks, T. ; Prins, T.W. ; Stam, P. ; Visser, P.B. - \ 2005
Euphytica 145 (2005)3. - ISSN 0014-2336 - p. 339 - 347.
seedling-vigor - linkage map - traits - growth - potato - rflp - aflp - construction - resistance - markers
Breeding of cut and pot rose cultivars for efficient production under low-energy conditions in greenhouses will be facilitated by understanding the inheritance of vigour. To get insight into the genetic variation of vigour-related traits, a diploid rose population was employed for an evaluation study in greenhouses in The Netherlands and Denmark. For all the traits investigated the population showed a continuous quantitative variation as well as a considerable transgression. For most of the traits, the genetic variation found among the tested entries was highly significant and tended to be large in comparison to the effects of genotype by environment interaction. The heritability based on means of the traits was high and ranged from 68 to 92%. Strong simple correlations (r = 0.65 to 0.95) were found among the traits shoot length, leaf area, leaf dry weight, stem dry weight, total dry weight and growth rate. The total dry weight and leaf area are suggested to be good parameters for early selection of rose genotypes with vigorous growth under suboptimal growth conditions.
Concervus-mix : met heerlijke stukjes architectuur
Hondt, H. d'; Hendriks, T. - \ 2003
Topos : periodiek over landschapsarchitectuur, ruimtelijke planning en sociaal-ruimtelijke analyse 13 (2003)2. - ISSN 1572-302X - p. 35 - 37.
landschapsarchitectuur - natuurbescherming - beroepsopleiding - landscape architecture - nature conservation - vocational training
Korte impressie van het afstudeervak "wild uit het vuistje; over echte natuur in een oerhollands landschap"