Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Kennisimpuls Bestuivers
Groot, G.A. de; Laros, I. ; Roessink, I. - \ 2018
Wageningen University & Research
Developing forensic tools for an African timber : Regional origin is revealed by genetic characteristics, but not by isotopic signature
Vlam, Mart ; Groot, Arjen de; Boom, Arnoud ; Copini, Paul ; Laros, Ivo ; Veldhuijzen, Katrui ; Zakamdi, David ; Zuidema, Pieter A. - \ 2018
Biological Conservation 220 (2018). - ISSN 0006-3207 - p. 262 - 271.
DNA - Geographic origin - Microsatellites - Stable isotopes - Timber forensics - Tropical timber
Combatting illegal timber trade requires forensic tools that independently verify claimed geographic origin of timber. Chemical and genetic wood characteristics are potentially suitable tools, but their performance at small spatial scales is unknown. Here we test whether stable isotopes and microsatellites can differentiate Tali timber (Erythrophleum spp.) at the level of forest concessions. We collected 394 wood samples from 134 individuals in five concessions in Cameroon and Congo Republic. The nearest neighbour concessions were 14 km apart and the furthest pair 836 km apart. We constructed genetic profiles using eight nuclear microsatellite markers and measured concentrations of δ18O, δ15N and δ13C. We differentiated provenances using PCA (microsatellites), ANOVA and kernel discriminant analysis (isotopes). Next, we performed assignment tests using blind samples (n = 12, microsatellites) and leave one out cross validation (LOOCV, isotopes). Isotopic composition varied strongly within concessions and only δ13C differed significantly between two concessions. As a result, LOOCV performed only marginally better than random. Genetic differentiation among provenances was also relatively low, but private alleles were commonly found. Bayesian clustering analysis correctly assigned 92% of the blind samples, including those of nearby concessions. Thus, Tali timber can be successfully assigned to the concession of origin using genetic markers, but not using isotopic composition. Isotopic differentiation may be possible at larger spatial scales or with stronger climatic or topographic variation. Our study shows that genetic analyses can differentiate the geographic origin of tropical timber at the scale of forest concessions, demonstrating their potential as forensic tools to enforce timber trade legislation.
The need for standardisation : Exemplified by a description of the diversity, community structure and ecological indices of soil nematodes
Griffiths, B.S. ; Groot, G.A. de; Laros, I. ; Stone, D. ; Geisen, S. - \ 2018
Ecological Indicators 87 (2018). - ISSN 1470-160X - p. 43 - 46.
Biodiversity - DNA extraction - Metabarcoding - Microscopy - Molecular approaches - Nematodes - Standardisation
Molecular approaches are offering a supplement to, or even the possibility of replacing morphological identification of soil fauna, because of advantages for throughput, coverage and objectivity. We determined ecological indices of nematode community data from four sets of duplicate soil cores, based on morphological identification of nematodes after elutriation from 200 g soil and high throughput sequencing (HTS) targeting nematodes both after being elutriated from soils and DNA extracted directly from 10 g soil. HTS (at genus and species level) increased the taxonomic resolution compared to morphology (at family level). DNA extracted from elutriated nematodes identified more nematode taxa than when extracted from soil, due to an enrichment in nematode sequences. Each method also gave a different ecological footprint for the nematode community. Standardisation to previously determined indices based on morphological identification is needed in order to provide more meaningful information about soil quality and for ecological monitoring.
Artic Aliens: Mapping the presence of marine alien species in west Svalbard
Heuvel-Greve, M.J. van den; Brink, A.M. van den; Steenhuisen, Frits ; Klaassen, Michiel ; Heuvel, Mare van den; Glorius, S.T. ; Laros, I. ; Bovenschen, J. ; Groot, G.A. de - \ 2017
Globally, alien species are considered the third most important threat to biodiversity after habit loss and fishery. Alien species have the potential to impact the environment and economy by disrupting the ecological system. To better understand what species are, or could be introduced to the Arctic, it is necessary to have a fast, efficient and accurate monitoring method for identifying alien species. We developed and tested a DNA technique that allows an easy detection of the presence of multiple species in a water or sediment sample.
Arctic Aliens: mapping the presence of marine alien species in west Svalbard
Heuvel-Greve, M.J. van den; Brink, A.M. van den; Steenhuisen, Frits ; Klaassen, Michiel ; Heuvel, M. van den; Glorius, S.T. ; Laros, I. ; Bovenschen, J. ; Groot, G.A. de - \ 2017
Globally, alien species are considered the third most important threat to biodiversity after habitat loss and fishery. Alien species have the potential to impact the environment and economy by disrupting the ecological system. To better understand what species are, or could be introduced to the Arctic, it is necessary to have a fast, efficient and accurate monitoring method for identifying alien species. We developed and tested a DNA technique that allows an easy detection of the presence of multiple species in a water or sediment sample.
Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding
Groot, G.A. de; Geisen, Stefan ; Wubs, E.R.J. ; Laros, I. ; Meulenbroek, Liz ; Kuiters, A.T. ; Slim, P.A. - \ 2016
Understanding variation in airborne biological dispersal is relevant not only to understand biogeographical patterns, but also because various biological particles may be allergenic or pathogenic to plants or animals (including humans). Weather conditions will influence both the release and spread of biological propagules in the air, and thus the composition of the airborne community (the ‘aerobiome’). Molecular techniques are increasingly used to study airborne dispersal of bacteria and fungi, although often including a culturing step. Other life forms, mostly plants, have mainly been studied by analysing samples under the microscope. This limits the spatiotemporal scope and the ability to directly link the dispersal of flora and fauna to that of microbes.
We used DNA metabarcoding based on three genetic markers to study the entire aerobiome in direct DNA extracts from air samples taken over a continuous period of 21 days at two different sites on the Wageningen Campus. We observed a large diversity of microbes (bacteria, fungi and protists) as well as plants. Using multivariate and network analyses, we studied relations between the presence of taxa from different branches of the tree of life, and explored how community shifts related to meteorological parameters and life history traits.
We show that communities were dominated by spore forming fungi and protists, many of those resembling (plant) pathogens. Relations with weather conditions varied among taxa. Oomycetes for example, predominantly containing (plant) pathogens, occurred in much higher relative abundances at low air humidity, while many fungi and plants were still abundant on very rainy days. These types of observations may help to explore potential effects of climatic changes on species distributions and the spread of pests and diseases.
Herkomst en migratie van Nederlandse edelherten en wilde zwijnen : een basiskaart van de genetische patronen in Nederland en omgeving
Groot, G.A. de; Spek, G.J. ; Bovenschen, J. ; Laros, I. ; Meel, Tom van; Jansman, H.A.H. - \ 2016
Wageningen : Alterra, Wageningen-UR (Alterra-rapport 2724) - 71 p.
cervus elaphus - sus scrofa - migration - genetic variation - wildlife conservation - netherlands - migratie - genetische variatie - wildbescherming - nederland
De laatste jaren worden in toenemende mate incidentele waarnemingen van edelherten en wilde zwijnen buiten de toegewezen leefgebieden gedaan. De vraag is vervolgens of dit om natuurlijke immigratie vanuit (niet-omrasterde) leefgebieden in binnen- of buitenland gaat en waar ze dan vandaan zijn gekomen, of dat het een ontsnapt of losgelaten dier betreft. Om deze vraag in de toekomst in voorkomende gevallen effectief te kunnen beantwoorden, stelde Alterra in opdracht van BIJ12 – Faunafonds en Vereniging Het Edelhert een landelijke genetische referentiedatabase op van de zwijnen- en edelhertenpopulaties in Nederland en nabijgelegen populaties in België en Duitsland. In dit rapport worden de mogelijkheden van deze databases voor herkomstbepalingen nader onderzocht. Tevens geeft dit onderzoek, op basis van de verkregen databases, een overzicht van de genetische vitaliteit van de Nederlandse populaties van beide soorten met betrekking tot diversiteit, inteeltrisico’s en uitwisselingsmogelijkheden.
Genetische diversiteit van de door essentaksterfte geplaagde gewone es
Copini, P. ; Kopinga, J. ; Laros, I. ; Bovenschen, J. ; Buiteveld, J. - \ 2016
Vakblad Natuur Bos Landschap (2016)april. - ISSN 1572-7610 - p. 14 - 17.
chalara fraxinea - fraxinus excelsior - schimmelziekten - resistentie van variëteiten - genetische diversiteit - rassenlijsten - genenbanken - bosbeheer - fungal diseases - varietal resistance - genetic diversity - descriptive list of varieties - gene banks - forest administration
Essenzaadbronnen op de Rassenlijst Bomen hebben een hoge genetische diversiteit. Dat is een belangrijk kwaliteitsaspect van teeltmateriaal en speelt een cruciale rol in het aanpassingsvermogen van soorten aan een veranderend klimaat en nieuwe ziekten en plagen. De huidige generatie essenbossen heeft te kampen met de veelal desastreuze gevolgen van essentaksterfte. Uit onderzoek blijkt dat resistentie tegen deze ziekte genetisch bepaald is, en dat een klein deel van de essen goed bestand is tegen de ziekte. Deze genetische basis biedt hoop om een
nieuwe generatie essen te selecteren die bestand is tegen de ziekte, met behoud van de genetische diversiteit.
EcoFINDERS soil protist metabarcoding
Geisen, Stefan ; Laros, I. ; Vizcaino, A. ; Bonkowski, M. ; Groot, G.A. de - \ 2015
PRJEB9172
We created a mock community of common free-living soil protists (amoebae, flagellates, ciliates), extracted DNA and amplified it in the presence of metazoan DNA using 454 HTS. We aimed at evaluating whether HTS quantitatively reveals true relative abundances of soil protists and to investigate whether the expected protist community structure is altered by the co-amplification of metazoan-associated protist taxa. Indeed, HTS revealed fundamentally different protist communities from those expected. Ciliate sequences were highly overrepresented, while those of most amoebae and flagellates were underrepresented or totally absent. These results underpin the biases introduced by HTS that prevent reliable quantitative estimations of free-living protist communities. Furthermore, we detected a wide range of non-added protist taxa likely introduced along with metazoan DNA, which altered the protist community structure. Among those, 20 taxa most closely resembled parasitic, often pathogenic taxa.
Data from: Not all are free-living: high-throughput DNA metabarcoding reveals a diverse community of protists parasitizing soil metazoa
Geisen, Stefan ; Laros, I. ; Vizcaino, A. ; Bonkowski, M. ; Groot, G.A. de - \ 2015
protists - parasitology - worms - host parasite interactions - DNA barcoding
Protists, the most diverse eukaryotes, are largely considered to be free-living bacterivores, but vast numbers of taxa are known to parasitize plants or animals. High-throughput sequencing (HTS) approaches now commonly replace cultivation-based approaches in studying soil protists, but insights into common biases associated with this method are limited to aquatic taxa and samples. We created a mock community of common free-living soil protists (amoebae, flagellates, ciliates), extracted DNA and amplified it in the presence of metazoan DNA using 454 HTS. We aimed at evaluating whether HTS quantitatively reveals true relative abundances of soil protists and at investigating whether the expected protist community structure is altered by the co-amplification of metazoan-associated protist taxa. Indeed, HTS revealed fundamentally different protist communities from those expected. Ciliate sequences were highly over-represented, while those of most amoebae and flagellates were under-represented or totally absent. These results underpin the biases introduced by HTS that prevent reliable quantitative estimations of free-living protist communities. Furthermore, we detected a wide range of nonadded protist taxa probably introduced along with metazoan DNA, which altered the protist community structure. Among those, 20 taxa most closely resembled parasitic, often pathogenic taxa. Therewith, we provide the first HTS data in support of classical observational studies that showed that potential protist parasites are hosted by soil metazoa. Taken together, profound differences in amplification success between protist taxa and an inevitable co-extraction of protist taxa parasitizing soil metazoa obscure the true diversity of free-living soil protist communities.
Molecular identification of soil eukaryotes and focused approaches targeting protist and faunal groups using high-throughput metabarcoding
Groot, G.A. de; Laros, I. ; Geisen, S. - \ 2015
In: Microbial Environmental Genomics (MEG) / Martin, F., Uroz, S., Springer (Methods in Molecular Biology ) - ISBN 9781493933679 - p. 125 - 140.
While until recently the application of high-throughput sequencing approaches has mostly been restricted to bacteria and fungi, these methods have now also become available to less often studied (eukaryotic) groups, such as fauna and protists. Such approaches allow routine diversity screening for large numbers of samples via DNA metabarcoding. Given the enormous taxonomic diversity within the eukaryote tree of life, metabarcoding approaches targeting a single specific DNA region do not allow to discriminate members of all eukaryote clades at high taxonomic resolution. Here, we report on protocols that enable studying the diversity of soil eukaryotes and, at high taxonomic resolution, of individual faunal and protist groups therein using a tiered approach: first, the use of a general eukaryotic primer set targeting a wide range of eukaryotes provides a rough impression on the entire diversity of protists and faunal groups. Second, more focused approaches enable deciphering subsets of soil eukaryotes in higher taxonomic detail. We provide primers and protocols for two examples: soil microarthropods and cercozoan protists.
Not all are free-living: high-throughput DNA metabarcoding reveals a diverse community of protists parasitizing soil metazoa
Geisen, S. ; Laros, I. ; Vizcaino, A. ; Bonkowski, M. ; Groot, G.A. de - \ 2015
Molecular Ecology 24 (2015)17. - ISSN 0962-1083 - p. 4556 - 4569.
Protists, the most diverse eukaryotes, are largely considered to be free-living bacterivores, but vast numbers of taxa are known to parasitize plants or animals. High-throughput sequencing (HTS) approaches now commonly replace cultivation-based approaches in studying soil protists, but insights into common biases associated with this method are limited to aquatic taxa and samples. We created a mock community of common free-living soil protists (amoebae, flagellates, ciliates), extracted DNA and amplified it in the presence of metazoan DNA using 454 HTS. We aimed at evaluating whether HTS quantitatively reveals true relative abundances of soil protists and at investigating whether the expected protist community structure is altered by the co-amplification of metazoan-associated protist taxa. Indeed, HTS revealed fundamentally different protist communities from those expected. Ciliate sequences were highly over-represented, while those of most amoebae and flagellates were under-represented or totally absent. These results underpin the biases introduced by HTS that prevent reliable quantitative estimations of free-living protist communities. Furthermore, we detected a wide range of nonadded protist taxa probably introduced along with metazoan DNA, which altered the protist community structure. Among those, 20 taxa most closely resembled parasitic, often pathogenic taxa. Therewith, we provide the first HTS data in support of classical observational studies that showed that potential protist parasites are hosted by soil metazoa. Taken together, profound differences in amplification success between protist taxa and an inevitable co-extraction of protist taxa parasitizing soil metazoa obscure the true diversity of free-living soil protist communities.
Inteelt onder Sallandse korhoenders : de genetische gevolgen van een kleine populatieomvang
Groot, G.A. de; Jansman, H.A.H. ; Bovenschen, J. ; Laros, I. ; Meyer-Lucht, Y. ; Hoglund, J. - \ 2014
Wageningen : Alterra, Wageningen-UR (Alterra-rapport 2599) - 47
tetrao tetrix - geïntroduceerde soorten - fauna - dierecologie - habitats - genetisch evenwicht - salland - introduced species - animal ecology - genetic equilibrium
Recent werd Alterra gevraagd om de resultaten van onderzoek dat de afgelopen jaren door verschillende instituten is uitgevoerd te bundelen en te integreren om een antwoord te krijgen op de vraag of het korhoen duurzaam voor Nederland behouden kan worden en op welke manier behoud mogelijk is (Jansman et al. 2014). Het voorliggende rapport is een aanvulling op dit basisrapport, waarin dieper wordt ingegaan op de effecten die de sterke afname in populatieomvang heeft gehad op het verloop van genetische processen van de korhoenpopulatie, en de gevolgen die dit kan hebben voor de algehele vitaliteit van de populatie, en diens reproductievermogen in het bijzonder. In dit onderzoek is duidelijk geworden dat de Nederlandse korhoenpopulatie al in 2010 genetisch zeer sterk was verarmd. In 2013, toen slechts zeven individuen overgebleven waren, bleek de variatie nog verder teruggelopen, ook op genen die van belang zijn voor een goed functionerend immuunsysteem
A tiered approach for high-resolution characterization of the soil faunal community via dna metabarcoding
Groot, G.A. de; Geisen, S. ; Laros, I. ; Faber, J.H. ; Schmeltz, R. - \ 2014
In: Book of Abstracts of the First Global Soil Biodiversity Conference. - - p. 87 - 87.
Characterization of the huge soil faunal diversity still relies heavily on the slow and expertdependent morphological identification. This hampers our ecological understanding of spatial and temporal diversity of many faunal groups, as screening many samples at high taxonomic detail is not a realistic proposition. DNA-based approaches, especially high-throughput DNA metabarcoding assays, potentially solve this issue, but the development of such methods targeting soil fauna lags far behind that of soil microbes. Within the EU FP7-project EcoFINDERS, we developed and tested a framework for automated identification of six different groups of soil fauna at high taxonomic detail, with a single integrated method. We adopted a tiered approach, in which a general eukaryotic marker is used to screen for the presence of different eukaryotic clades and a set of more specific markers is simultaneously analyzed to obtain high resolution data for six different groups: mites, collembola, enchytraeids, nematodes, earthworms and protists. New primer sets, as well as reference barcode datasets were established for several of them. Here, we show the results of two test runs based on 454 pyrosequencing. In the first run, artificially created DNA pools of known composition were analysed to test to which extent the taxonomic composition could successfully be retrieved. Preliminary results show that for all groups the majority of species in the DNA pool were recovered by the metabarcoding approach. By comparing results for DNA pools that contained different relative amounts of DNA of the six groups, we could show that for most markers the number of taxa of the targeted group recovered depended on the presence of DNA from non-targeted groups. In the second run we moved towards the analysis of actual soil (e)DNA extracts, comparing the results of morphological identification by those of molecular identification based on the same soil samples.
Aquatische exoten vroeg detecteren via eDNA : case study rivierkreeften
Groot, G.A. de; Laros, I. ; Ottburg, F.G.W.A. ; Roesink, J.G.J. - \ 2014
Landschap : tijdschrift voor landschapsecologie en milieukunde 31 (2014)3. - ISSN 0169-6300 - p. 146 - 153.
aquatische ecosystemen - rivierkreeft - invasieve exoten - detectie - dna - innovaties - aquatic ecosystems - crayfish - invasive alien species - detection - innovations
Aquatische exoten laten kleine hoeveelheden DNA achter in hun leefmilieu, zogenaamd environmental DNA of eDNA. Daarmee kan de aanwezigheid van bepaalde exoten op een eenvoudige manier (analyse van watermonsters) worden aangetoond. Environmental DNA-detectiemethoden zijn echter nog volop in ontwikkeling. Aan de hand van exotische rivierkreeften laten we zien wat er komt kijken bij de ontwikkeling en validatie van een nieuwe eDNA-detectiemethode en bij het daadwerkelijk gebruiken van dergelijke methoden.
The first wolf found in the Netherlands in 150 years was the victim of a wildlife crime
Gravendeel, B. ; Groot, G.A. de; Kik, M. ; Beentjes, K. ; Bergman, H. ; Caniglia, R. ; Cremers, H. ; Fabbri, E. ; Groenenberg, D. ; Grone, A. ; Groot Bruinderink, G.W.T.A. ; Font, I. ; Hakhof, J. ; Harms, V. ; Jansman, H.A.H. ; Janssen, R. ; Lammertsma, D.R. ; Laros, I. ; Linnartz, L. ; Marel, D. van der; Mulder, J.L. ; Mije, S. van der; Nieman, A.M. ; Nowak, C. ; Randi, E. ; Rijks, M. ; Speksnijder, A. ; Vonhof, H.B. - \ 2013
Lutra 56 (2013)2. - ISSN 0024-7634 - p. 93 - 109.
wolven - fauna - migratie - diergedrag - dna - biochemie - menselijke invloed - centraal-europa - noordoostpolder - wolves - migration - animal behaviour - biochemistry - human impact - central europe
On July 4th 2013 a dead subadult female wolf-like canid was found by the roadside between Luttelgeest and Marknesse in the Noordoostpolder in the central part of the Netherlands. As the last observations of wild wolves in the Netherlands date back to 1869 the discovery of this animal generated a lot of media attention. European wolf populations have been expanding since the 1950s and the first packs recently established themselves in Germany in geographic proximity of the Dutch border, so natural re-appearance of the species in the Netherlands seemed likely. We investigated the taxonomy of the animal, its geographical origin, and its most recent history. Macroscopic and biochemical analyses of the dead animal convincingly showed that it was a purebred wolf, related to populations from eastern Europe. Bullet impacts and shattered fragments found in the chest and flank, and a discrepancy between the timing of the post mortem and rigor mortis intervals indicated that this wolf was shot prior to illegal transport to the Netherlands. The wolf fed on beaver in either the Carpathian mountains or the Eifel which is too far for the animal to have walked from by itself within the 24 hours needed to digest its last meal. These geographical areas are the only regions where haplotypes and 87Sr/86Sr isotopes retrieved from both the dead wolf and the beaver remains in its stomach co-occur. We therefore conclude that the first Dutch wolf found in the Netherlands in 150 years did not enter the Netherlands by itself but sadly proved to be the victim of wildlife crime. Keywords: Canis lupus, Europe, haplotypes, isotopes, microsatellites, wildlife forensics, wolf.
Genetica van wilde zwijnen in Limburg en Noord-Brabant : verspreiding, herkomst en verwantschap
Jansman, H.A.H. ; Hofmeester, T.R. ; Groot, G.A. de; Laros, I. ; Bovenschen, J. ; Speelman, M. ; Hout, J.J. van der; Casaer, J. ; Breyne, P. ; Koelewijn, H.P. - \ 2013
Wageningen : Alterra, Wageningen-UR (Alterra-rapport 2404) - 62
sus scrofa - wilde varkens - populatiedynamica - populatiegenetica - genetica - migratie - limburg - noord-brabant - fauna - wild pigs - population dynamics - population genetics - genetics - migration
In Limburg komen op verschillende plaatsen buiten het officiële leefgebied De Meinweg wilde zwijnen voor. De aantallen in deze zogenaamde nulstandsgebieden nemen toe, en ook in Noord-Brabant worden in toenemende mate wilde zwijnen gesignaleerd. In opdracht van de provincies Limburg en Noord-Brabant voerde Alterra een genetisch onderzoek uit naar de populatiestructuur en herkomst van de wilde zwijnen in Limburg en Noord-Brabant. Het onderzoek laat zien dat sprake is van een aantal afzonderlijke populaties met een unieke genetische samenstelling. Door gunstigere omstandigheden weten de populaties van wilde zwijnen in Noordwest Europa in aantal en verspreidingsgebied toe te nemen. Genetisch kon dan ook vastgesteld worden dan in de meeste gevallen de nieuwe populaties ontstaan zijn uit de naburige bestaande populaties. In enkele gevallen kan sprake zijn geweest van een (gedeeltelijke) onnatuurlijke oorsprong door ontsnappingen uit gevangenschap of illegale herintroducties. De genetische variatie in de populaties is redelijk, maar laag vergeleken met het buitenland. Onder de huidige omstandigheden lijkt de uitwisseling tussen deze jonge populaties echter voldoende om de variatie in de toekomst op z'n minst in stand te houden.
Genetische tools voor natuurbeheer: van het lab naar de praktijk
Groot, G.A. de; Laros, I. - \ 2013
Vakblad Natuur Bos Landschap 10 (2013)4. - ISSN 1572-7610 - p. 4 - 7.
natuurbeheer - genetische diversiteit - populatiegenetica - populatie-ecologie - nature management - genetic diversity - population genetics - population ecology
Genetische technieken worden nog altijd maar mondjesmaat gebruikt in het natuurbeheer en -beleid. Dit is deels het gevolg van een imagoprobleem: in het verleden waren deze technieken prijzig, en de toepassingsmogelijkheden beperkt. De moleculaire ecologie staat echter niet stil en nieuwe ontwikkelingen volgen elkaar in hoog tempo op. Gevestigde technieken hebben hun concrete waarde voor beleidsvorming en praktisch populatiebeheer inmiddels bewezen, en werden prijstechnisch steeds interessanter. Nieuwe technieken bieden een efficïënt alternatief voor conventionele inventarisaties. Tijd voor een update.
De Nederlandse populatie van Geelbuikvuurpad in Zuid-Limburg: een genetische analyse
Jansman, H.A.H. ; Groot, G.A. de; Speelman, M. ; Laros, I. ; Bosman, W. ; Crombaghs, B. ; Koelewijn, H.P. - \ 2012
Wageningen : Alterra, Wageningen-UR - 55 p.
Assessment 2012 Alterra, klantenonderzoek
Boonstra, F. ; Arnouts, R.C.M. ; Dirksen, J. ; Fontein, R.J. ; Knoppersen, S. ; Laros, I. ; Nieuwenhuizen, W. - \ 2012
Wageningen : Alterra, Wageningen-UR - 44
milieubescherming - natuurbescherming - wetenschappelijk onderzoek - kennisoverdracht - inventarisaties - environmental protection - nature conservation - scientific research - knowledge transfer - inventories
Klanten van Alterra oordelen positief over de relatie met Alterra en de bruikbaarheid van het Alterra-onderzoek van de afgelopen vijf jaar. Ze gebruiken onderzoeksresultaten instrumenteel, conceptueel, strategisch en relationeel. Verbeterpunten voor Alterra liggen op het vlak van meer interne en externe samenwerking, het versterken van de politiek-bestuurlijke gevoeligheid van de onderzoekers en projectmatig werken. Ook roepen klanten Alterra op kritisch te blijven op de inhoudelijke kwaliteit van het onderzoek. Kansen zien zij in nieuwe rollen van onderzoekers zoals die van actie-onderzoeker en kennismakelaar.
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