Birth, death and horizontal transfer of the fumonisin biosynthetic gene cluster during the evolutionary diversification of Fusarium
Proctor, R.H. ; Hove, F. van; Susca, A. ; Stea, G. ; Busman, M. ; Lee, T.A.J. van der; Waalwijk, C. ; Moretti, A. ; Ward, T.J. - \ 2013
Molecular Microbiology 90 (2013)2. - ISSN 0950-382X - p. 290 - 306.
fujikuroi species complex - maximum-likelihood - gibberella-moniliformis - aspergillus-parasiticus - secondary metabolism - comparative genomics - adaptive evolution - multigene family - section liseola - mixed models
Fumonisins are a family of carcinogenic secondary metabolites produced by members of the Fusarium fujikuroi species complex (FFSC) and rare strains of Fusarium oxysporum. In Fusarium, fumonisin biosynthetic genes (FUM) are clustered, and the cluster is uniform in gene organization. Here, sequence analyses indicated that the cluster exists in five different genomic contexts, defining five cluster types. In FUM gene genealogies, evolutionary relationships between fusaria with different cluster types were largely incongruent with species relationships inferred from primary-metabolism (PM) gene genealogies, and FUM cluster types are not trans-specific. In addition, synonymous site divergence analyses indicated that three FUM cluster types predate diversification of FFSC. The data are not consistent with balancing selection or interspecific hybridization, but they are consistent with two competing hypotheses: (i) multiple horizontal transfers of the cluster from unknown donors to FFSC recipients and (ii) cluster duplication and loss (birth and death). Furthermore, low levels of FUM gene divergence in F.¿bulbicola, an FFSC species, and F.¿oxysporum provide evidence for horizontal transfer of the cluster from the former, or a closely related species, to the latter. Thus, uniform gene organization within the FUM cluster belies a complex evolutionary history that has not always paralleled the evolution of Fusarium
Gibberella musae (Fusarium musae) sp. nov., a recently discovered species from banana is sister to F. verticillioides
Hove, F. van; Waalwijk, C. ; Logrieco, A. ; Munaut, F. ; Moretti, A. - \ 2011
Mycologia 103 (2011)3. - ISSN 0027-5514 - p. 570 - 585.
fujikuroi mating population - pcr-rflp analysis - section liseola - fumonisin production - prairie grasses - gene-cluster - complex - anamorph - sorghum - differentiation
Several strains of Fusarium isolated from banana were identified previously as F. verticillioides (Sacc.) Nirenberg but described as unable to produce fumonisin. Here we report biochemical and morphological evidence, as well as multilocus phylogenetic analyses based on elongation factor (EF-1a), calmodulin, b-tubulin, and the second largest subunit of RNA polymerase II (RPB2) sequences, indicating that these isolates represent a unique lineage in the Gibberella fujikuroi species complex related to but distinct from F. verticillioides. Together with previous results of molecular studies, as well as with results of metabolite analyses, crossing experiments, pathogenicity tests and morphological characterization, these new data indicate that these strains isolated from banana represent a new species, Gibberella musae Van Hove et al. sp. nov. (anamorph: Fusarium musae Van Hove et al. sp. nov.), which is described herein
Variation in sequence and location of the fumonisin mycotoxin niosynthetic gene cluster in Fusarium
Proctor, R.H. ; Hove, F. van; Susca, A. ; Stea, A. ; Busman, M. ; Lee, T.A.J. van der; Waalwijk, C. ; Moretti, A. - \ 2010
Gewasbescherming 41 (2010)6. - ISSN 0166-6495 - p. 296 - 296.
plantenvirussen - fusarium - genetische variatie - mycotoxinen - fumonisinen - genetische analyse - plant viruses - genetic variation - mycotoxins - fumonisins - genetic analysis
In Fusarium, the ability to produce fumonisins is governed by a 17-gene fumonisin biosynthetic gene (FUM) cluster. Here, we examined the cluster in F. oxysporum strain O-1890 and nine other species selected to represent a wide range of the genetic diversity within the GFSC.